Identification of miRNAs from stem cell derived microparticles in umbilical cord blood.

Authors :: Xagorari A
Gerousi M
Sioga A
Bougiouklis D
Argiriou A
Anagnostopoulos A
Sotiropoulos D
Source :: Experimental hematology [Exp Hematol] 2019 Dec; Vol. 80, pp. 21-26. Date of Electronic Publication: 2019 Nov 14.
Publication Year :: 2019
Abstract: Umbilical cord blood CD34+ (UCB-CD34+) stem cells are clinically used in hematopoietic cell transplantation. However, there are limitations in the use of umbilical cord blood transplants because of the small number of cells and delayed engraftment. To gain a better understanding of functional components of UCB, we have detected and characterized CD34+ microparticles (CD34+MPs) from cord blood units. We collected cord blood units and assessed the numbers of CD34+MPs before and after red blood cell and plasma depletion by SEPAX processing using flow cytometry analysis. In parallel we identified MPs by electron microscopy. CD34+MPs and cells were isolated by MACs sorting. MicroRNAs (miR-106, miR-221, miR-517, miR-519, and miR-221) exhibited a characteristic microRNA profile that was further validated in isolated CD34+MPs. We found that in cord blood, there are CD34+MPs that carry microRNAs.<br /> (Copyright © 2019 ISEH -- Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved.)

Subjects :: Annexin A5 analysis
Antigens, CD34 analysis
Hematopoietic Stem Cells ultrastructure
Humans
Infant, Newborn
MicroRNAs isolation & purification
Microscopy, Electron
Real-Time Polymerase Chain Reaction
Cell-Derived Microparticles chemistry
Cord Blood Stem Cell Transplantation methods
Fetal Blood chemistry
Hematopoietic Stem Cells chemistry
MicroRNAs blood

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