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Extracellular vesicles derived from Echinococcus granulosus hydatid cyst fluid from patients: isolation, characterization and evaluation of immunomodulatory functions on T cells.
- Source :
-
International journal for parasitology [Int J Parasitol] 2019 Dec; Vol. 49 (13-14), pp. 1029-1037. Date of Electronic Publication: 2019 Nov 14. - Publication Year :
- 2019
-
Abstract
- Cystic echinococcosis is a chronic and complex zoonotic disease. The mechanisms underlying the parasite's establishment, growth and persistence are not completely understood, and are thought be modulated by a crosstalk through extracellular vesicles. Here, EVs were isolated from the hydatid cyst fluid of patients with cystic echinococcosis and protoscolex culture supernatant. Proteomic analysis of these EVs revealed several parasite- and human-derived proteins. Very few studies have performed proteomic analysis of EVs isolated from HCF and PCS. Our proteomic analysis of the EVs derived from HCF and PCS facilitated identification of 1175 proteins, wherein 1026 and 38 proteins were exclusively identified in the EVs derived from HCF (HCF-EVs) and PCS (PCS-EVs), respectively, and 111 proteins were shared in both. The results of co-culture of PCS-EVs with murine peripheral blood mononuclear cells showed that PCS-EVs significantly regulated T lymphocyte functions in a dose-dependent manner. Collectively, our results provide valuable information on parasite survival strategies and new insights into the role of these EVs in the establishment and persistence of hydatid cysts.<br /> (Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Subjects :
- Animals
Cyst Fluid chemistry
Extracellular Vesicles chemistry
Host-Parasite Interactions
Humans
Immune Evasion
Mice
Proteome analysis
T-Lymphocytes drug effects
Cyst Fluid immunology
Echinococcosis parasitology
Echinococcus granulosus growth & development
Echinococcus granulosus immunology
Extracellular Vesicles immunology
Immunologic Factors analysis
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0135
- Volume :
- 49
- Issue :
- 13-14
- Database :
- MEDLINE
- Journal :
- International journal for parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 31734339
- Full Text :
- https://doi.org/10.1016/j.ijpara.2019.08.003