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[Molecular mechanism of macrophages derived from PGRN gene knockout mice inhibit invasion and migration of breast cancer cells].
- Source :
-
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2019 Sep; Vol. 35 (9), pp. 769-775. - Publication Year :
- 2019
-
Abstract
- Objective To explore the functions and mechanisms of macrophages derived from PGRN gene knockout (PGRN <superscript>-/-</superscript> ) C57BL/6 mice in the invasion and migration of breast cancer cells. Methods Breast cancer cells were cultured in conditioned medium of macrophages derived from WT and PGRN <superscript>-/-</superscript> mice. Transwell <superscript>TM</superscript> assay and scratch assay were used to detect the invasion and migration ability of cancer cells. Western blot analysis was used to detect the expression of E-cadherin and N-cadherin in cancer cells. Cytokine array, real-time quantitative PCR and ELISA were performed to investigate the differences of cytokines secreted by macrophages derived from WT and PGRN <superscript>-/-</superscript> mice. Breast cancer cells were treated by the differentially expressed cytokine interleukin-6 (IL-6), and then the above methods were used to investigate its effect on cancer cells. Western blot analysis was used to verify the roles of NF-κB and JAK/STAT3 signaling pathways. Results The macrophages derived from PGRN <superscript>-/-</superscript> mice blocked NF-κB signaling pathway, reduced IL-6 secretion, and inhibited the invasion and migration of breast cancer cells. IL-6 activated JAK/STAT3 signaling pathway to promote the invasion and migration of breast cancer cells. Conclusion The macrophages derived from PGRN <superscript>-/-</superscript> mice can block the NF-κB and JAK/STAT3 signaling pathways, down-regulate IL-6 expression, and inhibit the invasion and migration of breast cancer cells.
- Subjects :
- Animals
Breast Neoplasms immunology
Cadherins
Cell Movement
Culture Media, Conditioned
Granulins
Mice
Mice, Inbred C57BL
Mice, Knockout
NF-kappa B metabolism
Neoplasm Invasiveness
Progranulins
STAT3 Transcription Factor metabolism
Tumor Cells, Cultured
Breast Neoplasms pathology
Intercellular Signaling Peptides and Proteins genetics
Interleukin-6 immunology
Macrophages immunology
Signal Transduction
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-8738
- Volume :
- 35
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 31750816