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Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2020 Feb 05; Vol. 68 (5), pp. 1207-1212. Date of Electronic Publication: 2019 Dec 06. - Publication Year :
- 2020
-
Abstract
- Human noroviruses (HuNoVs) are among the main pathogens causing acute nonbacterial gastroenteritis. Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding. HBGA-like substances in produce are also considered as the critical ligands for capture of HuNoVs. However, the composition of viral ligands from food substrates remains unknown. In this study, an oligosaccharide (H2N2F2) was captured and isolated from romaine lettuce extract by a bacterial surface display system. Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs. The composition was consistent with our ELISA results using a panel of monoclonal antibodies against HBGAs. Our results revealed a possible interaction mechanism between HuNoVs and romaine lettuce. Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.
- Subjects :
- Antigens, Plant chemistry
Antigens, Plant genetics
Blood Group Antigens chemistry
Blood Group Antigens genetics
Caliciviridae Infections genetics
Caliciviridae Infections metabolism
Caliciviridae Infections virology
Humans
Lactuca chemistry
Lactuca genetics
Lactuca metabolism
Mass Spectrometry
Norovirus genetics
Oligosaccharides chemistry
Oligosaccharides genetics
Oligosaccharides metabolism
Protein Binding
Receptors, Virus chemistry
Receptors, Virus genetics
Antigens, Plant metabolism
Blood Group Antigens metabolism
Lactuca virology
Norovirus physiology
Receptors, Virus metabolism
Virus Attachment
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 68
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31755264
- Full Text :
- https://doi.org/10.1021/acs.jafc.9b05887