Effects of current intensities on the performances and microbial communities in a combined bio-electrochemical and sulfur autotrophic denitrification (CBSAD) system.

The lab-scale system combined bioelectrochemical and sulfur autotrophic denitrification (CBSAD) was established to evaluate the effects of currents (50-300 mA) on both the performances and microbial communities. Results showed that the nitrate removal rate increased significantly when the current increased from 50 to 200 mA, while it slightly decreased with higher currents. Mass balance results revealed that hydrogen autotrophic denitrification contributed almost three times (70.25-78.62%) to denitrification compared with that of the sulfur part (21.38-29.75%). Illumina MiSeq sequencing showed that the currents changed the bacterial richness and diversity in this system. Phylum Firmicutes and class Clostridia predominated >50% under each condition. And multiple key bacteria capable of denitrification such as Proteiniclasticum, Thauera and Family_XI_uncultured were identified and found in higher proportions when the current was 200 mA. Therefore, this study helps revealing the mechanisms of accelerating nitrate-reduction through applied currents in the CBSAD systems.
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