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Three-dimensional cartilage tissue regeneration system harnessing goblet-shaped microwells containing biocompatible hydrogel.
- Source :
-
Biofabrication [Biofabrication] 2019 Dec 23; Vol. 12 (1), pp. 015019. Date of Electronic Publication: 2019 Dec 23. - Publication Year :
- 2019
-
Abstract
- Differentiation of stem cells into chondrocytes has been studied for the engineering of cartilage tissue. However, stem cells cultured two-dimensionally have limited ability to differentiate into chondrocytes, which led to the development of three-dimensional culture systems. A recently developed microtechnological method uses microwells as a tool to form uniformly sized spheroids. In this study, we fabricated an array (10 × 10) of goblet-shaped microwells based on polydimethylsiloxane for spheroid culture. A central processing unit (CPU) was used to form holes, and metallic beads were used to form hemispherical microwell geometry. The holes were filled with Pluronic F-127 to prevent cells from sinking through the holes and allowing the cells to form spheroids. Viability and chondrogenic differentiation of human adipose-derived stem cells were assessed. The fabrication method using a micro-pin mold and metallic beads is easy and cost-effective. Our three-dimensional spheroid culture system optimizes the efficient differentiation of cells and has various applications, such as drug delivery, cell therapy, and tissue engineering.
- Subjects :
- Cartilage chemistry
Cell Differentiation
Chondrocytes chemistry
Chondrocytes cytology
Chondrogenesis
Goblet Cells chemistry
Goblet Cells cytology
Hydrogels chemistry
Spheroids, Cellular chemistry
Spheroids, Cellular cytology
Stem Cells chemistry
Stem Cells cytology
Tissue Engineering instrumentation
Tissue Scaffolds chemistry
Cartilage cytology
Tissue Engineering methods
Subjects
Details
- Language :
- English
- ISSN :
- 1758-5090
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biofabrication
- Publication Type :
- Academic Journal
- Accession number :
- 31783391
- Full Text :
- https://doi.org/10.1088/1758-5090/ab5d3e