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K092A and K092B, Two Peptides Isolated from the Dogfish ( Scyliorhinus canicula L.), with Potential Antineoplastic Activity Against Human Prostate and Breast Cancer Cells.
- Source :
-
Marine drugs [Mar Drugs] 2019 Nov 28; Vol. 17 (12). Date of Electronic Publication: 2019 Nov 28. - Publication Year :
- 2019
-
Abstract
- Cancer therapy is currently a major challenge within the research community, especially in reducing the side effects of treatments and to develop new specific strategies against cancers that still have a poor prognosis. In this context, alternative strategies using biotechnologies, such as marine peptides, have been developed based on their promise of effectivity associated with a low toxicity for healthy cells. The purpose of the present paper is to investigate the active mechanism of two peptides that were isolated from the epigonal tissue of the lesser spotted dogfish Scyliorhinus canicula L., identified NFDTDEQALEDVFSKYG (K092A) and EAPPEAAEEDEW (K092B) on the in vitro growth inhibition of ZR-75-1 mammary carcinoma cells and MDA-Pca-2b prostate cancer cells. The effects of the peptides on cell proliferation and cell death mechanisms were studied by the flow cytometry and immunofluorescence microscopy approaches. The results have shown the onset of both K092A- and K092B-induced early cytoskeleton changes, and then cell cycle perturbations followed by non-apoptotic cell death. Moreover, impedance perturbation and plasma membrane perforation in ZR-75-1 K092A-treated cell cultures and autophagy inhibition in MDA-Pca-2b K092B-treated cells have been observed. In conclusion, these two bioactive peptides from dogfish exhibit antineoplastic activity on the human prostate and breast cancer cells in vitro.
- Subjects :
- Action Potentials drug effects
Animals
Autophagy drug effects
Breast Neoplasms metabolism
Cell Line, Tumor
Cell Survival drug effects
Male
Mitochondria drug effects
Mitochondria metabolism
Prostatic Neoplasms metabolism
Antineoplastic Agents pharmacology
Breast Neoplasms drug therapy
Dogfish
Peptides pharmacology
Prostatic Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1660-3397
- Volume :
- 17
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Marine drugs
- Publication Type :
- Academic Journal
- Accession number :
- 31795172
- Full Text :
- https://doi.org/10.3390/md17120672