Back to Search
Start Over
Effect of passive ultrasonic activation on microorganisms in primary root canal infection: a randomized clinical trial.
- Source :
-
Journal of applied oral science : revista FOB [J Appl Oral Sci] 2019 Nov 28; Vol. 28, pp. e20190100. Date of Electronic Publication: 2019 Nov 28 (Print Publication: 2020). - Publication Year :
- 2019
-
Abstract
- Objective: This clinical study sought to evaluate the effectiveness of passive ultrasonic activation (PUA) in eliminating microorganisms in primary endodontic infection (PEI) after instrumentation of root canals using microbiological culture and checkerboard DNA-DNA hybridization.<br />Methodology: Twenty root canals with PEI and apical periodontitis were selected. The root canals were instrumented and then randomly divided into 2 groups, according to the irrigation method: PUA and conventional needle irrigation (CNI). Microbiological samples were collected before instrumentation (S1), after instrumentation (S2) and after irrigation with 17% EDTA (S3). The samples were subjected to anaerobic culture technique and checkerboard DNA-DNA hybridization analysis.<br />Results: A statistically significant difference was found between CNI (23.56%) and PUA (98.37%) regarding the median percentage values for culturable bacteria reduction (p<0.05). In the initial samples, the most frequently detected species was S. constellatus (50%), and after root canal treatment was E. faecalis (50%).<br />Conclusion: Both treatments significantly decreased the number of bacterial species compared with the initial sample. However, no statistical difference in the total microbial load between PUA and CNI groups was detected. The number of cultivable anaerobic bacteria reduced significantly using PUA, and the bacterial composition and number of bacterial species after using either CNI or PUA was similar.
- Subjects :
- Adolescent
Adult
Analysis of Variance
Bacteria isolation & purification
Bacterial Load
Colony Count, Microbial
Cone-Beam Computed Tomography
DNA Probes
Female
Humans
Linear Models
Male
Middle Aged
Root Canal Irrigants therapeutic use
Root Canal Therapy methods
Sodium Hypochlorite therapeutic use
Therapeutic Irrigation instrumentation
Therapeutic Irrigation methods
Treatment Outcome
Ultrasonic Therapy methods
Young Adult
Dental Pulp Cavity microbiology
Periapical Periodontitis therapy
Root Canal Therapy instrumentation
Ultrasonic Therapy instrumentation
Subjects
Details
- Language :
- English
- ISSN :
- 1678-7765
- Volume :
- 28
- Database :
- MEDLINE
- Journal :
- Journal of applied oral science : revista FOB
- Publication Type :
- Academic Journal
- Accession number :
- 31800872
- Full Text :
- https://doi.org/10.1590/1678-7757-2019-0100