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[Long non-coding RNAs show different expression profiles and display competing endogenous RNA potential in placenta accreta spectrum disorders].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2019 Oct 30; Vol. 39 (10), pp. 1253-1259. - Publication Year :
- 2019
-
Abstract
- Objective: To investigate the expression profile of long non-coding RNAs (lncRNA) and identify potential lncRNA-related competing endogenous RNAs (ceRNA) in placenta accrete spectrum disorders (PAS).<br />Methods: Five tissue specimens of placental implantation and 5 adjacent normal placental tissues were collected from cesarean section deliveries complicated by PAS in our hospital between December, 2017 and June, 2018. Human microarrays were used to identify the lncRNAs that were differentially expressed in PAS, and 5 of the identified lncRNAs were further validated using qRT-PCR. GO and KEGG pathway analyses were performed to indentify the most significant enrichment functions. A ceRNA network was constructed based on ENST00000511361 (RP5-875H18.4), NR&#95;027457 (LINC00221) and NR&#95;126415 (FOXP4-AS1) to pinpoint the potential lncRNAs-related ceRNA.<br />Results: A total of 329 lncRNAs and 179 mRNAs were identified to have differential expression in PAS. The results of qRT-PCR were consistent with the human microarrays results. Transforming growth factor-β (TGF-β) signaling pathway was the most significantly enriched pathway. The constructed ceRNA network suggested that RP5-875H18.4--miRNA-218--SLIT2 had a potential ceRNA regulatory mechanism in PAS.<br />Conclusions: The differentially expressed lncRNAs are involved in the occurrence and progression of PAS possibly by regulating the TGF-β signaling pathway. The ceRNA network of RP5-875H18.4--miRNA-218--SLIT2 may play a role in the occurrence of PAS.
- Subjects :
- Cesarean Section
Female
Gene Regulatory Networks
Humans
Intercellular Signaling Peptides and Proteins genetics
MicroRNAs genetics
Nerve Tissue Proteins genetics
Placenta Accreta genetics
Pregnancy
Signal Transduction
Transforming Growth Factor beta
Placenta Accreta pathology
RNA, Long Noncoding genetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 39
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 31801721
- Full Text :
- https://doi.org/10.12122/j.issn.1673-4254.2019.10.19