Comparative study of HO-1 expressing synovial lining cells between RA and OA.

Authors :: Yang S
Ohe R
Aung NY
Kato T
Kabasawa T
Utsunomiya A
Takakubo Y
Takagi M
Yamakawa M
Source :: Modern rheumatology [Mod Rheumatol] 2021 Jan; Vol. 31 (1), pp. 133-140. Date of Electronic Publication: 2020 Jan 22.
Publication Year :: 2021
Abstract: Objectives: We aimed to clarify the characteristics of heme oxygenase (HO)-1 expressing cells in the synovium from rheumatoid arthritis (RA) and osteoarthritis (OA), and to investigate the co-expression of HO-1 and IgG-Fc/HLA-DR complex.<br />Methods: The characteristics of HO-1 expressing cells in the synovium were investigated by using immunohistochemistry. The co-expression of HO-1 and IgG-Fc/HLA-DR complex was examined by an in situ proximity ligation assay (PLA) with immunofluorescence. HO-1 mRNA was investigated by reverse transcription-polymerase chain reaction.<br />Results: The number of HO-1 <superscript>+</superscript> cells from the RA synovium is higher than that from OA synovium. The double positive cells of HO-1 and IgG-Fc/HLA-DR complex were detected by the in situ PLA with immunofluorescence in RA synovium. HO-1 mRNA was detected in both RA and OA synovium.<br />Conclusion: A portion of HO-1 <superscript>+</superscript> cells with IgG-Fc/HLA-DR complex in lining layer of RA may be concluded as one of antigen presenting cells in RA and may be involved in production of RF.