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Prognostic value of DNA ploidy and automated assessment of stroma fraction in prostate cancer.
- Source :
-
International journal of cancer [Int J Cancer] 2020 Aug 15; Vol. 147 (4), pp. 1228-1234. Date of Electronic Publication: 2020 Jan 02. - Publication Year :
- 2020
-
Abstract
- The combination of DNA ploidy and automatically estimated stroma fraction has been shown to correlate with recurrence and cancer death in colorectal cancer. We aimed to extend this observation and evaluate the prognostic importance of this combined marker in prostate cancer. DNA ploidy status was determined by image cytometry and the stroma fraction was estimated automatically on hematoxylin and eosin stained sections in three tumor samples from each patient to account for tumor heterogeneity. The optimal threshold for low (≤56%) and high (>56%) stroma fraction was identified in a discovery cohort (n = 253). The combined marker was validated in an independent patient cohort (n = 259) with biochemical recurrence as endpoint. The combined marker predicted biochemical recurrence independently in the validation cohort. Multivariable analysis showed that the highest risk of recurrence was observed for patients with samples that had both non-diploid ploidy status and a high stroma fraction (hazard ratio: 2.51, 95% confidence interval: 1.18-5.34). In conclusion, we suggest the combination of DNA ploidy and automatically estimated stroma fraction as a prognostic marker for the risk stratification of prostate cancer patients. It may also be a potential generic marker as concurrent results have been described in colorectal cancer.<br /> (© 2019 The Authors. International Journal of Cancer published by John Wiley & Sons Ltd on behalf of UICC.)
- Subjects :
- Aged
Biomarkers, Tumor metabolism
Cohort Studies
Flow Cytometry methods
Humans
Kaplan-Meier Estimate
Male
Middle Aged
Multivariate Analysis
Neoplasm Recurrence, Local
Prognosis
Prostatectomy
Prostatic Neoplasms genetics
Prostatic Neoplasms metabolism
Risk Factors
Automation, Laboratory methods
Biomarkers, Tumor genetics
DNA, Neoplasm genetics
Ploidies
Prostatic Neoplasms diagnosis
Staining and Labeling methods
Subjects
Details
- Language :
- English
- ISSN :
- 1097-0215
- Volume :
- 147
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 31846064
- Full Text :
- https://doi.org/10.1002/ijc.32832