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IL-36γ is a pivotal inflammatory player in periodontitis-associated bone loss.
- Source :
-
Scientific reports [Sci Rep] 2019 Dec 17; Vol. 9 (1), pp. 19257. Date of Electronic Publication: 2019 Dec 17. - Publication Year :
- 2019
-
Abstract
- Periodontitis is a prevalent chronic inflammatory disease due to the host response (IL-1β, IL-6, TNF-α and IL-17A) to oral bacteria such as Porphyromonas gingivalis. The newer members of the IL-1 family, IL-36s (IL-36α/IL-36β/IL-36γ/IL-36Ra/IL-38) are known to be involved in host defense against P. gingivalis in oral epithelial cells (OECs) and are considered as key inflammatory mediators in chronic diseases. The aim of this study was to investigate the potential role of IL-36s in periodontitis. We showed here that IL-36γ mRNA gingival expression is higher in periodontitis patients, whereas IL-36β and IL-36Ra mRNA expression are lower compared to healthy controls. Interestingly, the elevated IL-36γ expression in patients is positively correlated with the RANKL/OPG ratio, an index of bone resorption. In vitro, IL-36γ expression was induced through TLR2 activation in primary OECs infected with P. gingivalis but not in gingival fibroblasts, the most widespread cell type in gingival connective tissue. In OECs, recombinant IL-36γ enhanced the expression of inflammatory cytokines (IL-1β, IL-6, TNF-α and IL-36γ), of TLR2 and importantly, the RANKL/OPG ratio. These findings suggest that IL-36γ could be a pivotal inflammatory player in periodontitis by perpetuating gingival inflammation and its associated alveolar bone resorption and could be a relevant therapeutic target.
- Subjects :
- Cell Line
Female
Humans
Inflammation metabolism
Inflammation microbiology
Inflammation pathology
Male
Alveolar Bone Loss metabolism
Alveolar Bone Loss microbiology
Alveolar Bone Loss pathology
Bacteroidaceae Infections metabolism
Bacteroidaceae Infections pathology
Interleukin-1 metabolism
Periodontitis metabolism
Periodontitis microbiology
Periodontitis pathology
Porphyromonas gingivalis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 31848404
- Full Text :
- https://doi.org/10.1038/s41598-019-55595-9