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Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cell Culture Prevents UVB-Induced Skin Aging in Human Keratinocytes and Dermal Fibroblasts.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2019 Dec 19; Vol. 21 (1). Date of Electronic Publication: 2019 Dec 19. - Publication Year :
- 2019
-
Abstract
- Human adipose-derived mesenchymal stem cells-conditioned medium (ADSC-CM) contains cytokines and growth factors that can facilitate the regeneration and repair of various tissues and organs. In the present study, the protective activity of ADSC-CM treatment was investigated in UVB-irradiated human keratinocyte cell line HaCaTs and normal human dermal fibroblasts (NHDFs). It was found that ADSC-CM can modulate the expression of the signaling molecules in the early UVB responsive signaling pathways, including mitogen activated protein kinases (MAPKs), activator protein 1 (AP-1), and nuclear factor kappa B (NF-κB). In addition, ADSC-CM treatment could upregulate antioxidant response element (ARE) such as phase II gene heme oxygenase-1 (HO-1) and increase the expression of collagen synthesis enhancer gene transforming growth factor-β (TGF-β). The expression of matrix metalloproteinase-1 (MMP-1) and procollagen type I synthesis inhibitors such as interleukin-6 (IL-6) was also found to be suppressed upon ADSC-CM treatment. Taken together, our study illustrates the anti-photoaging activities of ADSC-CM in cell-based models.<br />Competing Interests: All authors declare no conflicts of interests.
- Subjects :
- Adipose Tissue metabolism
Cell Culture Techniques
Cells, Cultured
Cytokines metabolism
Fibroblasts cytology
Fibroblasts drug effects
Fibroblasts radiation effects
Gene Expression Regulation drug effects
Gene Expression Regulation radiation effects
Humans
Keratinocytes drug effects
Keratinocytes radiation effects
Matrix Metalloproteinase 1 metabolism
Mesenchymal Stem Cells metabolism
Signal Transduction drug effects
Signal Transduction radiation effects
Ultraviolet Rays adverse effects
Adipose Tissue cytology
Culture Media, Conditioned pharmacology
Keratinocytes cytology
Mesenchymal Stem Cells cytology
Skin Aging drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 21
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 31861704
- Full Text :
- https://doi.org/10.3390/ijms21010049