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Plasma Amine Oxidase-Induced Nanoparticle-to-Nanofiber Geometric Transformation of an Amphiphilic Peptide for Drug Encapsulation and Enhanced Bactericidal Activity.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2020 Jan 29; Vol. 12 (4), pp. 4323-4332. Date of Electronic Publication: 2020 Jan 14. - Publication Year :
- 2020
-
Abstract
- Patients with cancer have reduced immune function and are susceptible to bacterial infection after surgery, chemotherapy, or radiotherapy. Spherical nanoparticles formed by the self-assembled peptide V <subscript>6</subscript> K <subscript>3</subscript> can be used as carriers for poorly soluble antitumor drugs to effectively deliver drugs into tumor cells. V <subscript>6</subscript> K <subscript>3</subscript> was designed to achieve nanoparticle-to-nanofiber geometric transformation under induction by plasma amine oxidase (PAO). PAO is commercially available and functionally similar to lysyl oxidase (LO), which is widely present in serum. After the addition of fetal bovine serum (FBS) or PAO, the secondary structure of the peptide changed, while the spherical nanoparticles stretched and transformed into nanofibers. The conversion of the self-assembled morphology reveals the susceptibility of this amphiphilic peptide to subtle chemical modifications and may lead to promising strategies to control self-assembled architecture via enzyme induction. Enzymatically self-assembled V <subscript>6</subscript> K <subscript>3</subscript> had bactericidal properties after PAO addition that were surprisingly superior to those before PAO addition, enabling this peptide to be used to prevent infection. The amphiphilic peptide V <subscript>6</subscript> K <subscript>3</subscript> displayed antitumor properties and low toxicity in mammalian cells, demonstrating good biocompatibility, as well as bactericidal properties, to prevent bacterial contamination. These advantages indicate that enzymatically self-assembled V <subscript>6</subscript> K <subscript>3</subscript> has great biomedical application potential in cancer therapy.
- Subjects :
- Animals
HeLa Cells
Humans
Mice
NIH 3T3 Cells
Anti-Bacterial Agents chemistry
Anti-Bacterial Agents pharmacology
Antineoplastic Agents chemistry
Antineoplastic Agents pharmacology
Drug Carriers chemistry
Drug Carriers pharmacology
Monoamine Oxidase metabolism
Nanofibers chemistry
Nanofibers therapeutic use
Nanoparticles chemistry
Nanoparticles therapeutic use
Peptides chemistry
Peptides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 12
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 31899611
- Full Text :
- https://doi.org/10.1021/acsami.9b21296