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Differential innate immune responses induced by Mycoplasma hyopneumoniae and Mycoplasma hyorhinis in various types of antigen presenting cells.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2020 Jan; Vol. 240, pp. 108541. Date of Electronic Publication: 2019 Nov 29. - Publication Year :
- 2020
-
Abstract
- Mycoplasma (M.) hyopneumoniae is the etiological agent of enzootic pneumonia in pigs and is closely related to M. hyorhinis, which can be isolated from the healthy mucosal surfaces of the upper respiratory tract. In rare cases it can also cause arthritis and polyserositis. Since the innate immune system is an important first line of defense and promotes adaptive immune responses, we characterized the innate immune response of various antigen presenting cells (APCs) to M. hyopneumoniae and M. hyorhinis, which differ in their pathogenicity in vivo. Porcine peripheral blood mononuclear cells were infected with different multiplicities of infection (MOI) of live and inactivated porcine mycoplasmas. Both Mycoplasma species induced strong tumour necrosis factor (TNF) responses in monocytes, with a stronger activation by M. hyorhinis. This higher stimulatory activity was also confirmed for CD40 upregulation. Conventional and plasmacytoid dendritic cells (cDC and pDC, respectively) did not or poorly respond to mycoplasmas in terms of TNF expression but more efficiently in terms of CD40 upregulation. Again, these responses were generally stronger with M. hyorhinis than with M. hyopneumoniae. Both Mycoplasma species also activated B cells in terms of CD25 upregulation, proliferation, and IgM secretion. Interestingly, while the induction of CD25 and in particular proliferation was higher with M. hyorhinis, the IgM secretion did not differ between the two species with the exception of the highest dose of M. hyopneumoniae,which appeared to suppress IgM responses. Taken together, our results provide a comparative analysis of innate immune response with different porcine APCs and demonstrate Mycoplasma species-dependent differences, which could relate to their different pathogenicity in vivo.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Antigen-Presenting Cells microbiology
B-Lymphocytes immunology
CD40 Antigens genetics
CD40 Antigens immunology
Dendritic Cells immunology
Dendritic Cells microbiology
Immunoglobulin M analysis
Immunoglobulin M immunology
Interleukin-2 Receptor alpha Subunit genetics
Interleukin-2 Receptor alpha Subunit immunology
Leukocytes, Mononuclear microbiology
Mycoplasma Infections immunology
Mycoplasma Infections microbiology
Mycoplasma hyopneumoniae pathogenicity
Mycoplasma hyorhinis pathogenicity
Swine
Swine Diseases immunology
Swine Diseases microbiology
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha immunology
Antigen-Presenting Cells immunology
Immunity, Innate
Leukocytes, Mononuclear immunology
Mycoplasma Infections veterinary
Mycoplasma hyopneumoniae immunology
Mycoplasma hyorhinis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2542
- Volume :
- 240
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 31902489
- Full Text :
- https://doi.org/10.1016/j.vetmic.2019.108541