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Subcellular Organelle Toxicity Caused by Arsenic Nanoparticles in Isolated Rat Hepatocytes.
- Source :
-
The international journal of occupational and environmental medicine [Int J Occup Environ Med] 2020 Jan; Vol. 11 (1), pp. 41-52. - Publication Year :
- 2020
-
Abstract
- Background: Arsenic, an environmental pollutant, is a carcinogenic metalloid and also an anticancer agent.<br />Objective: To evaluate the toxicity of arsenic nanoparticles in rat hepatocytes.<br />Methods: Freshly isolated rat hepatocytes were exposed to 0, 20, 40, and 100 μM of arsenic nanoparticles and its bulk counterpart. Their viability, reactive oxygen species level, glutathione depletion, mitochondrial and lysosomal damage, and apoptosis were evaluated.<br />Results: By all concentrations, lysosomal damage and apoptosis were clearly evident in hepatocytes exposed to arsenic nanoparticles. Evaluation of mitochondria and lysosomes revealed that lysosomes were highly damaged.<br />Conclusion: Exposure to arsenic nanoparticles causes apoptosis and organelle impairment. The nanoparticles have potentially higher toxicity than the bulk arsenic. Lysosomes are highly affected. It seems that, instead of mitochondria, lysosomes are the first target organelles involved in the toxicity induced by arsenic nanoparticles.
- Subjects :
- Animals
Cells, Cultured
Glutathione metabolism
Hepatocytes cytology
Humans
Male
Mitochondria pathology
Oxidative Stress drug effects
Rats
Rats, Wistar
Reactive Oxygen Species metabolism
Apoptosis drug effects
Arsenic toxicity
Hepatocytes drug effects
Lysosomes pathology
Membrane Potential, Mitochondrial drug effects
Nanoparticles toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 2008-6814
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The international journal of occupational and environmental medicine
- Publication Type :
- Academic Journal
- Accession number :
- 31905194
- Full Text :
- https://doi.org/10.15171/ijoem.2020.1614