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Sleep pressure regulates mushroom body neural-glial interactions in Drosophila.
- Source :
-
Matters select [Matters Sel] 2019; Vol. 2019. Date of Electronic Publication: 2019 Mar 22. - Publication Year :
- 2019
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Abstract
- Sleep is a behavior that exists broadly across animal phyla, from flies to humans, and is necessary for normal brain function. Recent studies in both vertebrates and invertebrates have suggested a role for glial cells in sleep regulatory processes. Changes in neural-glial interactions have been shown to be critical for synaptic plasticity and circuit function. Here, we wanted to test the hypothesis that changes in sleep pressure alters neural-glial interactions. In the fruit fly, Drosophila melanogaster , sleep is known to be regulated by mushroom body (MB) circuits. We used the technique GFP Reconstitution Across Synaptic Partners (GRASP) to test whether changes in sleep pressure affect neural-glial interactions between MB neurons and astrocytes, a specialized glial cell type known to regulate sleep in flies and mammals. The MB-astrocyte GRASP signal was reduced after 24 h of sleep deprivation, whereas the signal returned to baseline levels following 72 h of recovery. Social enrichment, which increases sleep drive, similarly reduced the MB-astrocyte GRASP signal. We did not observe any changes in the MB-astrocyte GRASP signal over time-of-day, or following paraquat exposure or starvation. These data suggest that changes in sleep pressure are linked to dynamic changes in neural-glial interactions between astrocytes and neuronal sleep circuits, which are not caused by normal rest-activity cycles or stressors.
Details
- Language :
- English
- ISSN :
- 2297-9239
- Volume :
- 2019
- Database :
- MEDLINE
- Journal :
- Matters select
- Publication Type :
- Academic Journal
- Accession number :
- 31938713
- Full Text :
- https://doi.org/10.19185/matters.201903000008