A sensitive fluorescent sensor based on the photoinduced electron transfer mechanism for cefixime and ctDNA.

Cefixime is a third generation orally administered cephalosporin that is frequently used as a broad spectrum antibiotic against various gram-negative and gram-positive bacteria. In this study, a simple and sensitive fluorescent sensor for the determination of the cefixime and ctDNA was established based on the CdTe:Zn ²⁺ quantum dots (QDs). The fluorescence of CdTe:Zn ²⁺ QDs can be effectively quenched by cefixime in virtue of the surface binding of cefixime on CdTe:Zn ²⁺ QDs and the subsequent photoinduced electron transfer process from CdTe:Zn ²⁺ QDs to cefixime, in particular, the high sensitivity of QDs fluorescence emission to cefixime at the micromole per liter level, which render the cefixime-CdTe:Zn ²⁺ QDs system into fluorescence "OFF" status, then turn on in the presence of ctDNA. Furthermore, the Fourier transform infrared (FTIR) spectra of characteristic bands of C-N and N-H groups of cefixime endow evidence for the interaction of cefixime with CdTe:Zn ²⁺ QDs. The relative electrochemical behavior of the affinity of CdTe:Zn ²⁺ QDs for cefixime and ctDNA reveals the potential molecular binding mechanism.
(© 2020 John Wiley & Sons, Ltd.)