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Decreasing acute toxicity and suppressing colorectal carcinoma using Sorafenib-loaded nanoparticles.
- Source :
-
Pharmaceutical development and technology [Pharm Dev Technol] 2020 Jun; Vol. 25 (5), pp. 556-565. Date of Electronic Publication: 2020 Feb 02. - Publication Year :
- 2020
-
Abstract
- Objective: A polymer-based nanoparticle was constructed to target sorafenib delivery to colorectal carcinoma cells and decrease the side effects of the drug. Methods: Sorafenib-loaded nanoparticles (S-NPs) based on PEG-PLGA were prepared using a double emulsion solvent evaporation method. The properties of S-NPs were evaluated and then their effects on the viability of colorectal cancer cells and normal human cells were assessed. The mechanism of S-NP internalization was explored using cellular uptake assays and in vitro fluorescence confocal imaging. Acute toxicity of sorafenib on its own or within S-NPs was assessed in mice. Results: S-NPs showed high drug loading and entrapment efficiencies, they did not cause extensive hemolysis, and they efficiently inhibited growth of colorectal cancer cell lines and human umbilical vein endothelial cells. S-NPs showed lower acute toxicity than the free drug. Conclusions: Loading sorafenib into nanoparticles can enhance its uptake by colorectal cancer cells and decrease its acute toxicity.
- Subjects :
- Animals
Antineoplastic Agents administration & dosage
Antineoplastic Agents chemistry
Antineoplastic Agents toxicity
Apoptosis drug effects
Cell Line, Tumor
Cell Survival drug effects
Colorectal Neoplasms pathology
Drug Compounding
Endocytosis drug effects
Human Umbilical Vein Endothelial Cells
Humans
Lethal Dose 50
Mice
Mice, Inbred BALB C
Particle Size
Sorafenib administration & dosage
Sorafenib chemistry
Sorafenib toxicity
Toxicity Tests, Acute
Antineoplastic Agents pharmacology
Drug Carriers chemistry
Nanoparticles chemistry
Polyesters chemistry
Polyethylene Glycols chemistry
Sorafenib pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-9867
- Volume :
- 25
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Pharmaceutical development and technology
- Publication Type :
- Academic Journal
- Accession number :
- 31958240
- Full Text :
- https://doi.org/10.1080/10837450.2020.1718704