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Increasing the pyruvate pool by overexpressing phosphoenolpyruvate carboxykinase or triosephosphate isomerase enhances phloroglucinol production in Escherichia coli.
- Source :
-
Biotechnology letters [Biotechnol Lett] 2020 Apr; Vol. 42 (4), pp. 633-640. Date of Electronic Publication: 2020 Jan 21. - Publication Year :
- 2020
-
Abstract
- Objectives: Acetyl-CoA is a precursor for phloroglucinol (PG), and pyruvate is one of the sources of intracellular acetyl-CoA. Therefore, enhancing intracellular pyruvate levels may help to improve the anabolic pathway of PG.<br />Results: In this study, the effects of phosphoenolpyruvate carboxykinase (PckA, encoded by pckA) or triosephosphate isomerase (TpiA, encoded by tpiA) overexpression on the production of PG were studied. Overexpression of pckA or tpiA could enhance the pyruvate anabolic pathway in shake-flask culture compared to the control strain, and the concentration of PG also increased by 44% and 92%, respectively. In addition, the acetate levels were all down regulated by the overexpression of the two genes to some extent and lower acetate level resulted in lower ATP pool and higher survival rate.<br />Conclusions: These results indicate that overexpression of pckA or tpiA can enhance the pyruvate "pool" and PG production in Escherichia coli, which provides a new reference for further increasing the production of PG.
- Subjects :
- Batch Cell Culture Techniques instrumentation
Escherichia coli genetics
Escherichia coli Proteins genetics
Escherichia coli Proteins metabolism
Fermentation
Phosphoenolpyruvate Carboxykinase (ATP) genetics
Plasmids genetics
Transformation, Bacterial
Triose-Phosphate Isomerase genetics
Escherichia coli growth & development
Phloroglucinol metabolism
Phosphoenolpyruvate Carboxykinase (ATP) metabolism
Pyruvic Acid metabolism
Triose-Phosphate Isomerase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6776
- Volume :
- 42
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biotechnology letters
- Publication Type :
- Academic Journal
- Accession number :
- 31965395
- Full Text :
- https://doi.org/10.1007/s10529-020-02812-5