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ASIC1a channels regulate mitochondrial ion signaling and energy homeostasis in neurons.
- Source :
-
Journal of neurochemistry [J Neurochem] 2020 Apr; Vol. 153 (2), pp. 203-215. Date of Electronic Publication: 2020 Feb 19. - Publication Year :
- 2020
-
Abstract
- Acid-sensing ion channel 1a (ASIC1a) is well-known to play a major pathophysiological role during brain ischemia linked to acute acidosis of ~pH 6, whereas its function during physiological brain activity, linked to much milder pH changes, is still poorly understood. Here, by performing live cell imaging utilizing Na <superscript>+</superscript> and Ca <superscript>2+</superscript> sensitive and spatially specific fluorescent dyes, we investigated the role of ASIC1a in cytosolic Na <superscript>+</superscript> and Ca <superscript>2+</superscript> signals elicited by a mild extracellular drop from pH 7.4 to 7.0 and how these affect mitochondrial Na <superscript>+</superscript> and Ca <superscript>2+</superscript> signaling or metabolic activity. We show that in mouse primary cortical neurons, this small extracellular pH change triggers cytosolic Na <superscript>+</superscript> and Ca <superscript>2+</superscript> waves that propagate to mitochondria. Inhibiting ASIC1a with Psalmotoxin 1 or ASIC1a gene knockout blocked not only the cytosolic but also the mitochondrial Na <superscript>+</superscript> and Ca <superscript>2+</superscript> signals. Moreover, physiological activation of ASIC1a by this pH shift enhances mitochondrial respiration and evokes mitochondrial Na <superscript>+</superscript> signaling even in digitonin-permeabilized neurons. Altogether our results indicate that ASIC1a is critical in linking physiological extracellular pH stimuli to mitochondrial ion signaling and metabolic activity and thus is an important metabolic sensor.<br /> (© 2020 International Society for Neurochemistry.)
Details
- Language :
- English
- ISSN :
- 1471-4159
- Volume :
- 153
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31976561
- Full Text :
- https://doi.org/10.1111/jnc.14971