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Application of culturomics to characterize diverse anaerobic bacteria from the gastrointestinal tract of broiler chickens in relation to environmental reservoirs.
- Source :
-
Canadian journal of microbiology [Can J Microbiol] 2020 Apr; Vol. 66 (4), pp. 288-302. Date of Electronic Publication: 2020 Jan 27. - Publication Year :
- 2020
-
Abstract
- Characterization of the microbiota of chickens is of current interest. The goals of the current study were to apply anaerobic isolation methods to comprehensively isolate and identify bacteria from the gastrointestinal tract of chickens and their environment. Bacterial communities within the drinking water were dominated by Escherichia , whereas communities in litter were more representative of the cecum. The crop and small intestine (jejunum and ileum) were dominated by Lactobacillus and Enterococcus spp., and the cecum was dominated by Proteus spp. The collection of bacteria isolated was dominated by Enterococcus spp., Escherichia / Shigella spp., Lactobacillus spp., and Proteus spp.; however, many rare taxa were observed. These included members of the Clostridiales and Clostridium spp., which were commonly isolated from the ileum and cecum. Bacteria isolated by enrichment and direct plating differed. The selective de Man-Rogosa-Sharpe agar was commonly associated with the isolation of Lactobacillus spp. and yielded the lowest diversity of all methods utilized. Increased diversity and frequency of Clostridium spp. was observed in enrichments of blood and mucus or by plating on Columbia agar supplemented with 10% blood and gentamicin. The bacteria isolated from this study provide source material for genomic and functional studies in chicken hosts.
- Subjects :
- Animals
Bacteria classification
Bacteria genetics
Bacteria isolation & purification
Bacteria, Anaerobic classification
Bacteria, Anaerobic genetics
Cecum microbiology
Ileum microbiology
Phylogeny
Bacteria, Anaerobic isolation & purification
Chickens microbiology
Gastrointestinal Microbiome
Gastrointestinal Tract microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1480-3275
- Volume :
- 66
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Canadian journal of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 31986063
- Full Text :
- https://doi.org/10.1139/cjm-2019-0469