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Hyaluronic acid-decorated liposomal nanoparticles for targeted delivery of 5-fluorouracil into HT-29 colorectal cancer cells.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2020 Oct; Vol. 235 (10), pp. 6817-6830. Date of Electronic Publication: 2020 Jan 28. - Publication Year :
- 2020
-
Abstract
- The use of liposomes as drug carriers improves the therapeutic efficacy of anticancer drugs, while at the same time reducing side effects. Hyaluronic acid (HA) is recognized by the CD44 receptor, which is overexpressed in many cancer cells. In this study, we developed HA-modified liposomes encapsulating 5-fluorouracil (5-FU) and tested them against a CD44 expressing colorectal cell line (HT29) and a non-CD44 expressing hepatoma cell line. The average size of 5-FU-lipo and 5-FU-lipo-HA nanoparticles were 112 ± 28 and 144 ± 77 nm, respectively. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay showed selective cancer cell death depending on the CD44 expression in a time-dependent manner. Apoptosis assays and cell-cycle analysis indicated that G0/G1 arrest occurred. The colony formation study revealed that cells treated with 5-FU-lipo and 5-FU-lipo-HA had reduced colony formation. Quantitative reverse-transcription polymerase chain reaction study showed that the oncogenic messenger RNA and microRNA levels were significantly reduced in the 5-FU-lipo-HA-treated group, while tumor suppressors were increased in that group. We suggest that optimal targeted delivery and release of 5-FU into colorectal cancer cells, renders them susceptible to apoptosis, cell-cycle arrest, and decreased colony formation.<br /> (© 2020 Wiley Periodicals, Inc.)
- Subjects :
- Antineoplastic Agents chemistry
Antineoplastic Agents pharmacology
Apoptosis drug effects
Cell Cycle Checkpoints drug effects
Cell Line, Tumor
Colorectal Neoplasms metabolism
Drug Carriers chemistry
Drug Delivery Systems methods
G1 Phase drug effects
HT29 Cells
Hep G2 Cells
Humans
Hyaluronan Receptors metabolism
Particle Size
Resting Phase, Cell Cycle drug effects
Colorectal Neoplasms drug therapy
Fluorouracil chemistry
Fluorouracil pharmacology
Hyaluronic Acid chemistry
Liposomes chemistry
Nanoparticles chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4652
- Volume :
- 235
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 31989649
- Full Text :
- https://doi.org/10.1002/jcp.29576