Genome Mining and Biosynthesis of Primary Amine-Acylated Desferrioxamines in a Marine Gliding Bacterium.

Genome mining of Fulvivirga sp. W222 revealed a desferrioxamine-like biosynthetic gene cluster containing an unknown gene fulF that is conserved in many Bacteroidetes species. A series of primary amine-acylated desferrioxamine G ₁ analogues, fulvivirgamides, were identified, and fulvivirgamides A ₂ , B ₂ , B ₃ , and B ₄ ( 1 - 4 ) were purified and characterized. The function of FulF, which is a novel acyltransferase for the acylation of the primary amine of Desferrioxamine G ₁ , was verified by heterologous expression and feeding experiments.