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The QuantiFERON Monitor ® assay is predictive of infection post allogeneic hematopoietic cell transplantation.
- Source :
-
Transplant infectious disease : an official journal of the Transplantation Society [Transpl Infect Dis] 2020 Jun; Vol. 22 (3), pp. e13260. Date of Electronic Publication: 2020 Feb 29. - Publication Year :
- 2020
-
Abstract
- Introduction: Following allogeneic hematopoietic stem cell transplantation (alloHCT), excessive immunosuppression can be complicated by infection, while inadequate immunosuppression can result in graft-vs-host disease (GVHD). An accurate method to assess overall immune status post HCT is lacking. The QuantiFERON Monitor <superscript>®</superscript> (QFM) assay measures interferon gamma (IFN-γ) release from whole blood following incubation with both innate (Toll-like receptor 7, TLR7) and adaptive (CD3 antibody) stimulants and may result in a more complete assessment of the immune system.<br />Methods: Whole blood samples were prospectively collected from alloHCT recipients at conditioning followed by days 10, 30, 60, 90, 120, and 180 post-transplant and assayed by the QFM test. IFN-γ levels were correlated to time post HCT and episodes of infection and GVHD.<br />Results: Forty patients were enrolled in the study (68% male; median age 47 years; 58% matched related donors, 42% unrelated; 33% myeloablative). Post-stimulation IFN-γ levels rose steadily over the first 180 days post transplantation. IFN-γ levels were significantly lower in those with active infection compared to those without during the neutropenic period (P < .001). The assay was predictive of CMV reactivation (VL > 1000 copies/mL) post alloHCT (P = .001).<br />Conclusion: This is a promising assay to demonstrate immune recovery and predict risk of infection after alloHCT and may allow tailoring of immunosuppression, antimicrobial treatment, and prophylaxis.<br /> (© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Subjects :
- Adolescent
Adult
Aged
Female
Graft vs Host Disease prevention & control
Humans
Interferon-gamma Release Tests
Male
Middle Aged
Prospective Studies
Reagent Kits, Diagnostic
Reinfection diagnosis
Reinfection virology
Transplantation, Homologous adverse effects
Young Adult
Cytomegalovirus Infections diagnosis
Hematopoietic Stem Cell Transplantation adverse effects
Transplantation Conditioning adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1399-3062
- Volume :
- 22
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Transplant infectious disease : an official journal of the Transplantation Society
- Publication Type :
- Academic Journal
- Accession number :
- 32034973
- Full Text :
- https://doi.org/10.1111/tid.13260