Genotoxic Evaluation of Surfactin C in Chinese Hamster Lung Cell Line.

To investigate the mutation inducibility of surfactin C, we performed the chromosome aberration assay with Chinese hamster lung cells in vitro . The colorimetric MTT screening assay was carried out to determine the cytotoxicity index (IC ₅₀ ) of surfactin C. The IC ₅₀ value was 125 μg/ml. For the chromosome aberration test of surfactin C, the maximum concentration was employed as 125 μg/ml, followed by 62.5 and 31.25 μg/ml for the lower concentrations, with or without metabolic activation (S9). Cyclophosphamide and mitomycin C were used as positive controls in the presence and absence of S9 metabolic activation, respectively. These results showed that surfactin C was not capable of inducing chromosome aberration, as measured by the chromosome aberration test using Chinese hamster lung cell line. There is no evidence for surfactin C to have a genotoxic potential.
(© Korean Society of Toxicology 2009.)