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Recombinant elongation factor 1 alpha of Haemonchus contortus affects the functions of goat PBMCs.
- Source :
-
Parasite immunology [Parasite Immunol] 2020 May; Vol. 42 (5), pp. e12703. Date of Electronic Publication: 2020 Feb 28. - Publication Year :
- 2020
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Abstract
- Excretory/secretory proteins of Haemonchus contortus (HcESPs) intermingle comprehensively with host immune cells and modulate host immune responses. In this study, H contortus ES antigen named as elongation factor 1 alpha (HcEF-1α) was cloned and expressed. The influences of recombinant HcEF-1α on multiple functions of goat peripheral blood mononuclear cells (PBMCs) were observed in vitro. Immunoblot analysis revealed that rHcEF-1α was recognized by the serum of goat infected with H contortus. Immunofluorescence analysis indicated that rHcEF-1α was bound on surface of PBMCs. Moreover, the productions of IL-4, TGF-β1, IFN-γ and IL-17 of cells were significantly modulated by the incubation with rHcEF-1α. The production of interleukin IL-10 was decreased. Cell migration, cell proliferation and cell apoptosis were significantly increased; however, nitric oxide production (NO) was significantly decreased. The MHC II molecule expression of cells incubated with rHcEF-1α was increased significantly, whereas MHC-I was not changed as compared to the control groups (PBS control and pET32a). These findings indicated that rHcEF-1α protein might play essential roles in functional regulations of HcESPs on goat PBMC and mediate the immune responses of the host during host-parasite relationship.<br /> (© 2020 The Authors. Parasite Immunology published by John Wiley & Sons Ltd.)
- Subjects :
- Animals
Apoptosis
Cell Movement
Cell Proliferation
Goat Diseases genetics
Goat Diseases immunology
Goat Diseases physiopathology
Goats
Haemonchiasis immunology
Haemonchiasis parasitology
Haemonchiasis physiopathology
Haemonchus genetics
Helminth Proteins genetics
Interleukin-17 genetics
Interleukin-17 immunology
Nitric Oxide immunology
Peptide Elongation Factor 1 genetics
Goat Diseases parasitology
Haemonchiasis veterinary
Haemonchus immunology
Helminth Proteins immunology
Leukocytes, Mononuclear immunology
Peptide Elongation Factor 1 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-3024
- Volume :
- 42
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Parasite immunology
- Publication Type :
- Academic Journal
- Accession number :
- 32043596
- Full Text :
- https://doi.org/10.1111/pim.12703