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AMP-Kinase Dysfunction Alters Notch Ligands to Impair Angiogenesis in Neonatal Pulmonary Hypertension.
AMP-Kinase Dysfunction Alters Notch Ligands to Impair Angiogenesis in Neonatal Pulmonary Hypertension.
- Source :
-
American journal of respiratory cell and molecular biology [Am J Respir Cell Mol Biol] 2020 Jun; Vol. 62 (6), pp. 719-731. - Publication Year :
- 2020
-
Abstract
- Decreased angiogenesis contributes to persistent pulmonary hypertension of the newborn (PPHN); mechanisms remain unclear. AMPK (5'AMP activated protein kinase) is a key regulator of cell metabolism. We investigated the hypothesis that a decrease in AMPK function leads to mitochondrial dysfunction and altered balance of notch ligands delta-like 4 (DLL4) and Jagged 1 (Jag1) to impair angiogenesis in PPHN. Studies were done in fetal lambs with PPHN induced by prenatal ductus arteriosus constriction and gestation-matched control lambs. PPHN lambs were treated with saline or AMPK agonist metformin. Angiogenesis was assessed in lungs with micro-computed tomography angiography and histology. AMPK function; expression of mitochondrial electron transport chain (ETC) complex proteins I-V, Dll4, and Jag1; mitochondrial number; and in vitro angiogenesis function were assessed in pulmonary artery endothelial cells (PAEC) from control and PPHN lambs. AMPK function was decreased in PPHN PAEC and lung sections. Expression of mitochondrial transcription factor, PGC-1α, ETC complex proteins I-V, and mitochondrial number were decreased in PPHN. In vitro angiogenesis of PAEC and capillary number and vessel volume fraction in the lung were decreased in PPHN. Expression of DLL4 was increased and Jag1 was decreased in PAEC from PPHN lambs. AMPK agonists A769662 and metformin increased the mitochondrial complex proteins and number, in vitro angiogenesis, and Jag1 levels and decreased DLL4 levels in PPHN PAEC. Infusion of metformin in vivo increased the vessel density in PPHN lungs. Decreased AMPK function contributes to impaired angiogenesis in PPHN by altered balance of notch ligands in PPHN.
- Subjects :
- AMP-Activated Protein Kinase Kinases
Animals
Animals, Newborn
Biphenyl Compounds
Ductus Arteriosus embryology
Ductus Arteriosus surgery
Electron Transport
Enzyme Activation
Female
Hypertension, Pulmonary physiopathology
Ligands
Lung pathology
Metformin pharmacology
Metformin therapeutic use
Mitochondria metabolism
Neovascularization, Pathologic drug therapy
Persistent Fetal Circulation Syndrome drug therapy
Persistent Fetal Circulation Syndrome pathology
Persistent Fetal Circulation Syndrome physiopathology
Phosphorylation
Pregnancy
Protein Kinases physiology
Pyrones pharmacology
Sheep
Thiophenes pharmacology
Threonine metabolism
Transfection
Endothelial Cells enzymology
Hypertension, Pulmonary enzymology
Intracellular Signaling Peptides and Proteins metabolism
Jagged-1 Protein metabolism
Membrane Proteins metabolism
Neovascularization, Pathologic enzymology
Persistent Fetal Circulation Syndrome enzymology
Protein Kinases metabolism
Receptors, Notch metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1535-4989
- Volume :
- 62
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- American journal of respiratory cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 32048878
- Full Text :
- https://doi.org/10.1165/rcmb.2019-0275OC