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The Phenotype and Functional Activity of Mesenchymal Stromal Cells in Pediatric Patients with Non-Malignant Hematological Diseases.
- Source :
-
Cells [Cells] 2020 Feb 12; Vol. 9 (2). Date of Electronic Publication: 2020 Feb 12. - Publication Year :
- 2020
-
Abstract
- As the biology of mesenchymal stromal cells (MSCs) in patients with non-malignant hematological diseases (NMHD) is poorly understood, in the current study we performed a basic characterization of the phenotype and functional activity of NMHD-MSCs. Bone marrow (BM) of patients with thalassemia major (TM) possessed a significantly higher number of nucleated cells (BM-MNCs)/mL BM than healthy donors ( P < 0.0001), which however did not result in a higher number of colony forming units-fibroblast (CFU-F) per milliliter BM. In contrast, from 1 × 10 <superscript>6</superscript> BM-MNCs of patients with sickle cell disease (SCD) were generated significantly more CFU-Fs than from TM-BM-MNCs ( P < 0.013) and control group ( P < 0.02). In addition, NMHD-MSCs expressed significantly lower levels of CD146 molecule, demonstrated an equal proliferation potential and differentiated along three lineages (osteoblasts, chondrocytes and adipocytes) as healthy donors' MSCs, with exception of TM-MSCs which differentiated weakly in adipocytes. In contrast to other NMHD-MSCs and healthy donors' MSCs, TM-MSCs demonstrated an impaired in vitro immunosuppressive potential, either. Noteworthy, the majority of the immunosuppressive effect of NMHD-MSCs was mediated through prostaglandin-E2 (PGE2), because indomethacin (an inhibitor of PGE2 synthesis) was able to significantly reverse this effect. Our results indicate therefore that NMHD-MSCs, except TM-MSCs, may be used as an autologous cell-based therapy for post-transplant complications such as graft failure, graft-versus-host disease (GvHD) and osteonecrosis.<br />Competing Interests: The authors declare no competing interests.
- Subjects :
- Adipocytes cytology
Adipocytes metabolism
Adolescent
Anemia, Sickle Cell metabolism
CD146 Antigen metabolism
Case-Control Studies
Cell Differentiation drug effects
Cell Lineage
Cell Proliferation drug effects
Child
Child, Preschool
Chondrocytes cytology
Chondrocytes metabolism
Dinoprostone metabolism
Female
Humans
Indomethacin pharmacology
Male
Mesenchymal Stem Cells cytology
Osteoblasts cytology
Osteoblasts metabolism
Phenotype
beta-Thalassemia metabolism
Anemia, Sickle Cell pathology
Mesenchymal Stem Cells metabolism
beta-Thalassemia pathology
Subjects
Details
- Language :
- English
- ISSN :
- 2073-4409
- Volume :
- 9
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cells
- Publication Type :
- Academic Journal
- Accession number :
- 32059574
- Full Text :
- https://doi.org/10.3390/cells9020431