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Identification of a gene encoding the last step of the L-rhamnose catabolic pathway in Aspergillus niger revealed the inducer of the pathway regulator.
- Source :
-
Microbiological research [Microbiol Res] 2020 Feb 07; Vol. 234, pp. 126426. Date of Electronic Publication: 2020 Feb 07. - Publication Year :
- 2020
- Publisher :
- Ahead of Print
-
Abstract
- In fungi, L-rhamnose (Rha) is converted via four enzymatic steps into pyruvate and L-lactaldehyde, which enter central carbon metabolism. In Aspergillus niger, only the genes involved in the first three steps of the Rha catabolic pathway have been identified and characterized, and the inducer of the pathway regulator RhaR remained unknown. In this study, we identified the gene (lkaA) involved in the conversion of L-2-keto-3-deoxyrhamnonate (L-KDR) into pyruvate and L-lactaldehyde, which is the last step of the Rha pathway. Deletion of lkaA resulted in impaired growth on L-rhamnose, and potentially in accumulation of L-KDR. Contrary to ΔlraA, ΔlrlA and ΔlrdA, the expression of the Rha-responsive genes that are under control of RhaR, were at the same levels in ΔlkaA and the reference strain, indicating the role of L-KDR as the inducer of the Rha pathway regulator.<br /> (Copyright © 2020 Elsevier GmbH. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1618-0623
- Volume :
- 234
- Database :
- MEDLINE
- Journal :
- Microbiological research
- Publication Type :
- Academic Journal
- Accession number :
- 32062364
- Full Text :
- https://doi.org/10.1016/j.micres.2020.126426