Back to Search
Start Over
Involvement of Satellite Cell Activation via Nitric Oxide Signaling in Ectopic Orofacial Hypersensitivity.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2020 Feb 13; Vol. 21 (4). Date of Electronic Publication: 2020 Feb 13. - Publication Year :
- 2020
-
Abstract
- : The mechanical head-withdrawal threshold (MHWT) was significantly reduced following inferior alveolar nerve transection (IANX) in rats. Nitrate and nitrite synthesis was dramatically increased in the trigeminal ganglion (TG) at 6 h after the IANX. The relative number of neuronal nitric oxide synthase (nNOS)-immunoreactive (IR) cells was significantly higher in IANX rats compared to sham-operated and N-propyl-L-arginine (NPLA)-treated IANX rats. On day 3 after NPLA administration, the MHWT recovered considerably in IANX rats. Following L-arginine injection into the TG, the MHWT was significantly reduced within 15 min, and the mean number of TG cells encircled by glial fibrillary acidic protein (GFAP)-IR cells was substantially higher. The relative number of nNOS-IR cells encircled by GFAP-IR cells was significantly increased in IANX rats. In contrast, after NPLA injection into the TG, the relative number of GFAP-IR cells was considerably reduced in IANX rats. Fluorocitrate administration into the TG significantly reduced the number of GFAP-IR cells and prevented the MHWT reduction in IANX rats. The present findings suggest that following IANX, satellite glial cells are activated via nitric oxide (NO) signaling from TG neurons. The spreading satellite glial cell activation within the TG results in mechanical hypersensitivity of face regions not directly associated with the trigeminal nerve injury.<br />Competing Interests: The authors declare no competing interest.
- Subjects :
- Animals
Arginine analogs & derivatives
Arginine pharmacology
Disease Models, Animal
Humans
Hyperalgesia genetics
Hyperalgesia metabolism
Hyperalgesia pathology
Mandibular Nerve metabolism
Mandibular Nerve pathology
Mandibular Nerve Injuries drug therapy
Mandibular Nerve Injuries metabolism
Mandibular Nerve Injuries pathology
Neuralgia drug therapy
Neuralgia metabolism
Neuralgia pathology
Neuroglia metabolism
Rats
Rats, Sprague-Dawley
Satellite Cells, Skeletal Muscle drug effects
Signal Transduction genetics
Trigeminal Ganglion drug effects
Trigeminal Ganglion pathology
Trigeminal Nerve Injuries genetics
Trigeminal Nerve Injuries metabolism
Trigeminal Nerve Injuries pathology
Glial Fibrillary Acidic Protein genetics
Nitric Oxide genetics
Nitric Oxide Synthase Type I genetics
Satellite Cells, Skeletal Muscle metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 21
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 32070010
- Full Text :
- https://doi.org/10.3390/ijms21041252