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Diazepam binding inhibitor-like immunoreactivity (DBI-LI) in human CSF. Correlations with neurological disorders.
- Source :
-
Journal of the neurological sciences [J Neurol Sci] 1988 Nov; Vol. 87 (2-3), pp. 327-49. - Publication Year :
- 1988
-
Abstract
- Cerebrospinal fluid (CSF) levels of the anxiogenic neuropeptide diazepam binding inhibitor (DBI) were determined by radioimmunoassay in 281 patients who underwent evaluation for neurological problems. Serial dilution curves and reverse-phase high pressure liquid chromatography showed that the immunoreactive material in CSF behaved just as authentic DBI extracted from human brain. Furthermore in the assay there was no evidence of interference from CSF samples deprived of DBI by immunoaffinity. In 82 patients with no evidence of major lesions in the central nervous system, who acted as controls, the CSF DBI content was shown to be age- and sex-related. No correlation was observed with the CSF protein concentration. In patients with different types of dementia, the levels of CSF DBI were significantly increased in a group with normal pressure hydrocephalus. No significant differences were found between Alzheimer's disease, multi-infarct dementia, or dementia with Parkinson's disease and controls. In non-demented patients with Parkinson's disease the levels of DBI were increased in a subgroup with depressive disturbances whereas no differences was observed in the non-depressed cases. The content of DBI was markedly reduced in 5 cases with olivopontocerebellar atrophy and in 4 with spinocerebellar ataxia. In all the other disorders studied the levels of DBI were similar to or slightly lower (multiple sclerosis) than those of the controls. The origin of DBI in cerebrospinal fluid is uncertain; a number of various possibilities are discussed concerning the proposed role of DBI as modulator of brain GABAergic transmission.
- Subjects :
- Adolescent
Adult
Age Factors
Aged
Cerebellar Diseases cerebrospinal fluid
Diazepam Binding Inhibitor
Female
Freezing
Humans
Male
Middle Aged
Multiple Sclerosis cerebrospinal fluid
Nervous System Diseases physiopathology
Neuropeptides isolation & purification
Sex Factors
Tissue Preservation
Nervous System Diseases cerebrospinal fluid
Neuropeptides cerebrospinal fluid
Subjects
Details
- Language :
- English
- ISSN :
- 0022-510X
- Volume :
- 87
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- Journal of the neurological sciences
- Publication Type :
- Academic Journal
- Accession number :
- 3210041
- Full Text :
- https://doi.org/10.1016/0022-510x(88)90257-2