Evaluation of Cytotoxic Activity Alkaloid Fractions of Zanthoxylum acanthopodium DC. Fruits.

Authors :: Syari DM
Rosidah R
Hasibuan PAZ
Haro G
Satria D
Source :: Open access Macedonian journal of medical sciences [Open Access Maced J Med Sci] 2019 Nov 14; Vol. 7 (22), pp. 3745-3747. Date of Electronic Publication: 2019 Nov 14 (Print Publication: 2019).
Publication Year :: 2019
Abstract: Aim: This study was carried out to investigate cytotoxic activity towards T47D, 4T1, MCF-7, HeLa, and Raji cells of alkaloid fractions of Zanthoxylum acanthopodium DC. fruits. Zanthoxylum acanthopodium DC.<br />Methods: The fruit was extracted by maceration. The ethanol extract was fractionated with liquid-liquid extraction using n-hexane, chloroform at pH 3, 7, and 9 to obtained alkaloid fractions. Cytotoxic activity for fraction chloroform at pH 7 and 9 was determined with MTT assay.<br />Results: The IC <subscript>50</subscript> of fraction chloroform at pH 7 and 9 was (92.67 ± 1.37; 71.87 ± 1.04; 159.87 ± 0.63; 123.39 ± 0.81; and 103.09 ± 0.58 µg/mLfor pH 7) and (451.29 ± 25.48; 247.18 ± 2.82; 318.46 ± 5.40; 303.96 ± 8.75; and 181.45 ± 1.35 µg/mL for pH 9) respectively.<br />Conclusion: The results reveal that alkaloid fractions at pH 7 and 9 of Zanthoxylum acanthopodium DC. Fruits have cytotoxic activity. Our further study is to isolate and assesses anticancer activity from alkaloid compounds.<br /> (Copyright: © 2019 Dina Maya Syari, Rosidah Rosidah, Poppy Anjelisa Zaitun Hasibuan, Ginda Haro, Denny Satria.)

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