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Characteristics and complete genome sequence of the virulent Vibrio alginolyticus phage VAP7, isolated in Hainan, China.
- Source :
-
Archives of virology [Arch Virol] 2020 Apr; Vol. 165 (4), pp. 947-953. Date of Electronic Publication: 2020 Mar 04. - Publication Year :
- 2020
-
Abstract
- A novel Vibrio alginolyticus phage, VAP7, was isolated from seawater collected from Sanya, Hainan province, China. Whole-genome sequencing analysis revealed that phage VAP7 has a linear, double-stranded DNA genome of 144,685 bp with an average G+C content of 41.9% and a high degree of sequence similarity to Vibrio phage VP-1. Annotation results identified 193 open reading frames and one transfer RNA-encoding gene in the phage genome. The morphology and the results of phylogenetic analysis suggest that VAP7 should be classified as a new member of the family Ackermannviridae. Moreover, phage VAP7 grew over a wide pH (5.0-10.0) and temperature (4-40 °C) range. Host-range experiments revealed that VAP7 could infect 31 Vibrio alginolyticus strains. Thus, VAP7 infecting Vibrio alginolyticus strains represents a potential new candidate for use in phage therapy.
- Subjects :
- Bacteriophages classification
Bacteriophages pathogenicity
Bacteriophages physiology
Base Composition
China
Genomics
Host Specificity
Open Reading Frames
Phylogeny
Podoviridae classification
Podoviridae genetics
Podoviridae pathogenicity
Seawater virology
Virulence
Bacteriophages genetics
Genome, Viral
Vibrio alginolyticus virology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 165
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 32130520
- Full Text :
- https://doi.org/10.1007/s00705-020-04535-4