Nocardioides euryhalodurans sp. nov., Nocardioides seonyuensis sp. nov. and Nocardioides eburneiflavus sp. nov., isolated from soil.

Three aerobic, rod-shaped actinobacterial strains, designated MMS17-SY117 ^T , MMS17-SY207-3 ^T and MMS17-SY213 ^T , were isolated from soil and their taxonomic positions were analysed using a polyphasic approach. The isolates showed best growth at 30 °C, pH 7 and 0-1 % (w/v) NaCl. On the basis of 16S rRNA gene sequence similarity, the isolates were affiliated to the genus Nocardioides , and the closest species to MMS17-SY117 ^T , MMS17-SY207-3 ^T and MMS17-SY213 ^T were Nocardioides aestuarii JC2056 ^T (97.76%), Nocardioides currus IB-3 ^T (97.41%) and Nocardioides exalbidus RC825 ^T (98.71%), respectively. Each isolate formed a distinct cluster within the Nocardioides clade in the phylogenetic tree. The orthologous average nucleotide identity and digital DNA-DNA hybridization values were in the range of 74.4-85.7 % and 16.6-39.2 %, respectively, with the type strains of related species. The major polar lipids in all three strains were phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol. The predominant fatty acids were iso-C _16 : 0 and C _17 : 1  ω8 c . MK-8(H ₄ ) was the major isoprenoid quinone and ll-DAP was the major diamino acid. Galactose, glucose and rhamnose were present in the whole-cell hydrolysate, and MMS17-SY213 ^T also contained mannose and ribose. The DNA G+C contents of MMS17-SY117 ^T , MMS17-SY207-3 ^T and MMS17-SY213 ^T were 72.2, 70.4 and 71.5 mol%, respectively. The phylogenetic, phenotypic and chemotaxonomic data supported the classification of each strain as representing a new species of Nocardioides , for which the names Nocardioides euryhalodurans sp. nov. (MMS17-SY117 ^T =KCTC 49175 ^T =JCM 32831 ^T ), Nocardioides seonyuensis sp. nov. (MMS17-SY207-3 ^T =KCTC 49176 ^T =JCM 32832 ^T ) and Nocardioides eburneiflavus sp. nov. (MMS17-SY213 ^T =KCTC 49177 ^T =JCM 32833 ^T ) are proposed accordingly.