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Cd9 Protects Photoreceptors from Injury and Potentiates Edn2 Expression.
- Source :
-
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2020 Mar 09; Vol. 61 (3), pp. 7. - Publication Year :
- 2020
-
Abstract
- Purpose: Cd9 is a tetraspanin membrane protein that plays various roles in tissue development and disease pathogenesis, especially in cancer, but the expression patterns and function of Cd9 in retinal development and disease are not well understood. We asked its roles during retinal photoreceptor degeneration by using CD9-knockout mice.<br />Methods: Cd9 knockout mice and rd1 mice were used to examine roles of Cd9 for progression of photoreceptor degeneration. Reverse transcription-polymerase chain reaction and immunohistochemistry were mainly used as analytical methods.<br />Results: Cd9 transcripts were only weakly expressed in retina at embryonic day 14, but its expression level subsequently increased and peaked at around postnatal day 12. In 6-week-old female mice derived retina, mRNA expression decreased slightly but was maintained at a significant level. Published RNA-sequencing data and immunohistochemistry indicated that Cd9 was expressed abundantly in Müller glia and weakly in other retinal neurons. Notably, when photoreceptors were damaged, Cd9 expression was increased in rod photoreceptors and decreased in Müller glia. Cd9 knockout mice retinas developed normally; however, once the retina suffered damage, degeneration of photoreceptors was more severe in Cd9 knockout retinas than control retinas. Induction of Edn2, which is known to protect against photoreceptor damage, was severely hampered. In addition, induction of Socs3, which is downstream of gp130 (Il6st), was weaker in Cd9 knockout retinas.<br />Conclusions: Taken together, these findings indicate that, although Cd9 was dispensable for normal gross morphological development, it protected rod photoreceptors and enhanced Edn2 expression, possibly through modulation of gp130 signaling.
- Subjects :
- Animals
Cytokine Receptor gp130 physiology
Ependymoglial Cells metabolism
Female
Gene Expression Regulation physiology
Mice, Inbred ICR
Mice, Knockout
RNA, Messenger genetics
Retina growth & development
Retina metabolism
Retinal Degeneration genetics
Retinal Degeneration metabolism
Retinal Degeneration pathology
Retinal Rod Photoreceptor Cells pathology
Signal Transduction physiology
Tetraspanin 29 deficiency
Tetraspanin 29 genetics
Endothelin-2 metabolism
Retinal Degeneration prevention & control
Retinal Rod Photoreceptor Cells metabolism
Tetraspanin 29 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1552-5783
- Volume :
- 61
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Investigative ophthalmology & visual science
- Publication Type :
- Academic Journal
- Accession number :
- 32150249
- Full Text :
- https://doi.org/10.1167/iovs.61.3.7