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Global transcriptomic analysis of Cronobacter sakazakii CICC 21544 by RNA-seq under inorganic acid and organic acid stresses.
- Source :
-
Food research international (Ottawa, Ont.) [Food Res Int] 2020 Apr; Vol. 130, pp. 108963. Date of Electronic Publication: 2019 Dec 29. - Publication Year :
- 2020
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Abstract
- Cronobacter sakazakii is a common foodborne pathogen that can tolerate various stress conditions. Acidic environment is a common stress condition encountered by bacteria in food processing and gastrointestinal digestion, including both inorganic and organic acids. In order to elucidate the Acid Tolerance Response (ATR) of C. sakazakii, we performed high-throughput RNA-seq to compare gene expression under hydrochloric acid and citric acid stresses. In this study, 107 differentially expressed genes (DEGs) were identified in both acids, of which 85 DEGs were functionally related to the regulation of acid tolerance. Multiple layers of mechanisms may be applied by C. sakazakii in response to acid stress: Firstly, in order to reduce excessive intracellular protons, C. sakazakii pumps them out through trans-membrane proteins or consumes them through metabolic reactions. Secondly, under acidic conditions, a large amount of reactive oxygen species and hydroxyl radicals accumulate in the cells, resulting in oxidative damage. C. sakazakii protects cells by up-regulating the antioxidant stress genes such as soxS and madB. Thirdly, C. sakazakii chooses energy efficient metabolic pathways to reduce energy consumption and maintain necessary processes. Finally, genes involved in chemotaxis and motility were differentially expressed to respond to different acidic conditions. This study systematically analyzed the acid-resistant mechanism of C. sakazakii under the stress of organic and inorganic acids, and provided a theoretical basis for better control of its contamination in food.<br />Competing Interests: Declaration of Competing Interest The authors declared that there is no conflict of interest.<br /> (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Subjects :
- Bacterial Proteins genetics
Bacterial Proteins metabolism
Cronobacter sakazakii genetics
Down-Regulation
Gene Expression Regulation, Bacterial drug effects
Gene Expression Regulation, Bacterial physiology
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, RNA
Transcriptome
Up-Regulation
Acids pharmacology
Cronobacter sakazakii drug effects
Cronobacter sakazakii metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-7145
- Volume :
- 130
- Database :
- MEDLINE
- Journal :
- Food research international (Ottawa, Ont.)
- Publication Type :
- Academic Journal
- Accession number :
- 32156398
- Full Text :
- https://doi.org/10.1016/j.foodres.2019.108963