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Identification and functional characterization of Penicillium marneffei major facilitator superfamily (MFS) transporters.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2020 Jul; Vol. 84 (7), pp. 1373-1383. Date of Electronic Publication: 2020 Mar 12. - Publication Year :
- 2020
-
Abstract
- Penicillium Marneffei: is a thermally dimorphic fungus that causes penicilliosis, and become the third-most-common opportunistic fungal infection in immunocompromised patients in Southeast Asia. Azoles and amphotericin B have been introduced for the treatment, however, it is important to investigate possible mechanisms of azole resistance for future treatment failure. We identified 177 putative MFS transporters and classified into 17 subfamilies. Among those, members of the Drug:H <superscript>+</superscript> antiporter 1 subfamily are known to confer resistance to antifungals. Out of 39 paralogs, three (encoded by PmMDR1, PmMDR2 , and PmMDR3 ) were heterologously overexpressed in S. cerevisiae AD∆ conferred resistance to various drugs and compounds including azoles, albeit to different degrees. Pm MDR1-expressing strain showed resistance to the broadest range of drugs, followed by the Pm MDR3, and Pm MDR2 conferred weak resistance to a limited range of drugs. We conclude that PmMDR1 and PmMDR3 , may be able to serve as multidrug efflux pumps.
- Subjects :
- ATP Binding Cassette Transporter, Subfamily B genetics
Amino Acid Sequence
Amphotericin B therapeutic use
Asia, Southeastern epidemiology
Candida albicans drug effects
Candida albicans metabolism
Drug Resistance, Fungal drug effects
Humans
Immunocompromised Host
Microbial Sensitivity Tests
Mycoses drug therapy
Mycoses epidemiology
Mycoses microbiology
Phylogeny
Saccharomyces cerevisiae drug effects
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins genetics
Talaromyces drug effects
Transcriptome
Triazoles therapeutic use
ATP Binding Cassette Transporter, Subfamily B chemistry
ATP Binding Cassette Transporter, Subfamily B metabolism
Amphotericin B pharmacology
Antifungal Agents pharmacology
Mycoses metabolism
Talaromyces metabolism
Triazoles pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 84
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32163007
- Full Text :
- https://doi.org/10.1080/09168451.2020.1732185