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The Noonan Syndrome Gene Lztr1 Controls Cardiovascular Function by Regulating Vesicular Trafficking.
- Source :
-
Circulation research [Circ Res] 2020 May 08; Vol. 126 (10), pp. 1379-1393. Date of Electronic Publication: 2020 Mar 16. - Publication Year :
- 2020
-
Abstract
- Rationale: Noonan syndrome (NS) is one of the most frequent genetic disorders. Bleeding problems are among the most common, yet poorly defined complications associated with NS. A lack of consensus on the management of bleeding complications in patients with NS indicates an urgent need for new therapeutic approaches.<br />Objective: Bleeding disorders have recently been described in patients with NS harboring mutations of LZTR1 (leucine zipper-like transcription regulator 1), an adaptor for CUL3 (CULLIN3) ubiquitin ligase complex. Here, we assessed the pathobiology of LZTR1-mediated bleeding disorders.<br />Methods and Results: Whole-body and vascular specific knockout of Lztr1 results in perinatal lethality due to cardiovascular dysfunction. Lztr1 deletion in blood vessels of adult mice leads to abnormal vascular leakage. We found that defective adherent and tight junctions in Lztr1 -depleted endothelial cells are caused by dysregulation of vesicular trafficking. LZTR1 affects the dynamics of fusion and fission of recycling endosomes by controlling ubiquitination of the ESCRT-III (endosomal sorting complex required for transport III) component CHMP1B (charged multivesicular protein 1B), whereas NS-associated LZTR1 mutations diminish CHMP1B ubiquitination. LZTR1-mediated dysregulation of CHMP1B ubiquitination triggers endosomal accumulation and subsequent activation of VEGFR2 (vascular endothelial growth factor receptor 2) and decreases blood levels of soluble VEGFR2 in Lztr1 haploinsufficient mice. Inhibition of VEGFR2 activity by cediranib rescues vascular abnormalities observed in Lztr1 knockout mice Conclusions: Lztr1 deletion phenotypically overlaps with bleeding diathesis observed in patients with NS. ELISA screening of soluble VEGFR2 in the blood of LZTR1 -mutated patients with NS may predict both the severity of NS phenotypes and potential responders to anti-VEGF therapy. VEGFR inhibitors could be beneficial for the treatment of bleeding disorders in patients with NS.
- Subjects :
- Animals
Blood Vessels abnormalities
Blood Vessels drug effects
Carcinoma, Lewis Lung metabolism
Carcinoma, Lewis Lung pathology
Disease Models, Animal
Endosomal Sorting Complexes Required for Transport metabolism
Endosomes genetics
Endosomes pathology
Endothelial Cells drug effects
Endothelial Cells pathology
Haploinsufficiency
HeLa Cells
Hemorrhage genetics
Hemorrhage pathology
Hemorrhage prevention & control
Humans
Lymphokines genetics
Lymphokines metabolism
Mice, Inbred C57BL
Mice, Knockout
Neovascularization, Pathologic
Noonan Syndrome drug therapy
Noonan Syndrome genetics
Noonan Syndrome pathology
Phosphorylation
Platelet-Derived Growth Factor genetics
Platelet-Derived Growth Factor metabolism
Protein Kinase Inhibitors pharmacology
Protein Transport
Quinazolines pharmacology
Signal Transduction
Transcription Factors deficiency
Transcription Factors genetics
Ubiquitination
Vascular Endothelial Growth Factor Receptor-2 antagonists & inhibitors
Vascular Endothelial Growth Factor Receptor-2 genetics
Vascular Malformations drug therapy
Vascular Malformations genetics
Vascular Malformations pathology
Blood Vessels metabolism
Endosomes metabolism
Endothelial Cells metabolism
Hemorrhage metabolism
Noonan Syndrome metabolism
Transcription Factors metabolism
Vascular Endothelial Growth Factor Receptor-2 metabolism
Vascular Malformations metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1524-4571
- Volume :
- 126
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Circulation research
- Publication Type :
- Academic Journal
- Accession number :
- 32175818
- Full Text :
- https://doi.org/10.1161/CIRCRESAHA.119.315730