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Comparison of the effects of thymoquinone and silymarin on the brain of rats having ischemia-reperfusion in the lower extremities.
- Source :
-
Annali italiani di chirurgia [Ann Ital Chir] 2020; Vol. 91, pp. 131-136. - Publication Year :
- 2020
-
Abstract
- Aim: We aimed to show whether ischemia reperfusion (I/R) injury causes damage on brain or not, and whether thymoquinone and silymarin, as antioxidant and anti-inflammatory herbs, have beneficial effects on this damage or not.<br />Methods: Forty Wistar albino rats were carried out and were randomized to 4 groups with equal numbers (n=10): sham group, implemented of only anesthesia; control group, implemented of anesthesia and I/R injury; silymarin group, implemented of anesthesia and I/R injury and treated with a dose of 200 milligram/kg silymarin ip and thymoquinone group, implemented of anesthesia and I/R injury and treated with a dose of 20 mg/kg thymoquinone. Serum lipid hydroperoxide (LOOH) and total free sulfhydryl (Sh) levels were determined. Light microscopy was used to evaluate histological changes in brain tissue.<br />Results: Serum LOOH levels (0.21 ± 0.04 for control group, 0.29 ± 0.01 for sham group, 0.23 ± 0.09 for silymarin group, 0.29 ± 0.09 for thymoquinone group) were significantly higher and Sh levels (10.74 ± 1.71 for control group, 6.82 ± 0.24 for sham group, 9.12 ± 1.04 for silymarin group, 8.41 ± 1.12 for thymoquinone group) were significantly lower in control, silymarin and thymoquinone groups compared to control group (p<0.05 for all). According to the histopathologic damage score assessment, it was seen that the damage decreased significantly in the silymarin and the thymoquinone groups.<br />Conclusion: We showed that tissue damage also occurs in brain following the ischemia reperfusion. It was shown that thymoquinone and silymarin is quite effective in preventing this damage.<br />Key Words: Brain, Hydroperoxide levels, Ischemia reperfusion injury, Sulfhydryl levels, Silymarin, Thymoquinone.
Details
- Language :
- English
- ISSN :
- 2239-253X
- Volume :
- 91
- Database :
- MEDLINE
- Journal :
- Annali italiani di chirurgia
- Publication Type :
- Academic Journal
- Accession number :
- 32180584