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HLA-B influences integrin beta-1 expression and pancreatic cancer cell migration.
- Source :
-
Experimental cell research [Exp Cell Res] 2020 May 15; Vol. 390 (2), pp. 111960. Date of Electronic Publication: 2020 Mar 16. - Publication Year :
- 2020
-
Abstract
- Human leukocyte antigen (HLA) class I molecules present antigenic peptides to cytotoxic T cells, causing lysis of malignant cells. Transplantation biology studies have implicated HLA class I molecules in cell migration, but there has been little evidence presented that they influence cancer cell migration, a contributing factor in metastasis. In this study, we examined the effect of HLA-B on pancreatic cancer cell migration. HLA-B siRNA transfection increased the migration of the S2-013 pancreatic cancer cells but, in contrast, reduced migration of the PANC-1 and MIA PaCa-2 pancreatic cancer cell lines. Integrin molecules have previously been implicated in the upregulation of pancreatic cancer cell migration, and knockdown of HLA-B in S2-013 cells heightened the expression of integrin beta 1 (ITGB1), but in the PANC-1 and MIA PaCa-2 cells HLA-B knockdown diminished ITGB1 expression. A transmembrane sequence in an S2-013 HLA-B heavy chain matches a corresponding sequence in HLA-B in the BxPC-3 pancreatic cancer cell line, and knockdown of BxPC-3 HLA-B mimics the effect of S2-013 HLA-B knockdown on migration. In total, our findings indicate that HLA-B influences the expression of ITGB1 in pancreatic cancer cells, with concurrent distinctions in transmembrane sequences and effects on the migration of the cells.<br />Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Cell Line, Tumor
Cell Proliferation
Focal Adhesion Kinase 1 genetics
Focal Adhesion Kinase 1 metabolism
HLA-B Antigens metabolism
Humans
Integrin alpha2 genetics
Integrin alpha2 metabolism
Integrin beta1 metabolism
Organ Specificity
Pancreas pathology
Protein Isoforms antagonists & inhibitors
Protein Isoforms genetics
Protein Isoforms metabolism
RNA, Small Interfering genetics
RNA, Small Interfering metabolism
Signal Transduction
Cell Movement genetics
Gene Expression Regulation, Neoplastic
HLA-B Antigens genetics
Integrin beta1 genetics
Pancreas metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2422
- Volume :
- 390
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Experimental cell research
- Publication Type :
- Academic Journal
- Accession number :
- 32194036
- Full Text :
- https://doi.org/10.1016/j.yexcr.2020.111960