Start Over

Yeast strain Debaryomyces hansenii for amelioration of arsenic stress in rice.

Authors :: Kaur J
Anand V
Srivastava S
Bist V
Tripathi P
Naseem M
Nand S
Anshu
Khare P
Srivastava PK
Bisht S
Srivastava S
Source :: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2020 Jun 01; Vol. 195, pp. 110480. Date of Electronic Publication: 2020 Mar 20.
Publication Year :: 2020
Abstract: Arsenic (As) is a serious threat for environment and human health. Rice, the main staple crop is more prone to As uptake. Bioremediation strategies with heavy metal tolerant rhizobacteria are well known. The main objective of the study was to characterize arsenic-resistant yeast strains, capable of mitigating arsenic stress in rice. Three yeast strains identified as Debaryomyces hansenii (NBRI-Sh2.11), Candida tropicalis (NBRI-B3.4) and Candida dubliniensis (NBRI-3.5) were found to have As reductase activity. D. hansenii with higher As tolerance has As expulsion ability as compared to other two strains. Inoculation of D. hansenii showed improved detoxification through scavenging of reactive oxygen species (ROS) by the modulation of SOD and APX activity under As stress condition in rice. Modulation of defense responsive gene (NADPH, GST, GR) along with arsR and metal cation transporter are the probable mechanism of As detoxification as evident with improved membrane (electrolyte leakage) stability. Reduced grain As (~40% reduction) due to interaction with D. hansenii (NBRI-Sh2.11) further validated it's As mitigation property in rice. To the best of our knowledge D. hansenii has been reported for the first time for arsenic stress mitigation in rice with improved growth and nutrient status of the plant.<br />Competing Interests: Declaration of competing interest The authors declared no conflict of interest.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)