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Localized delivery of ibuprofen via a bilayer delivery system (BiLDS) for supraspinatus tendon healing in a rat model.

Authors :
Taylor BL
Kim DH
Huegel J
Raja HA
Burkholder SJ
Weiss SN
Nuss CA
Soslowsky LJ
Mauck RL
Kuntz AF
Bernstein J
Source :
Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2020 Nov; Vol. 38 (11), pp. 2339-2349. Date of Electronic Publication: 2020 Apr 07.
Publication Year :
2020

Abstract

The high prevalence of tendon retear following rotator cuff repair motivates the development of new therapeutics to promote improved tendon healing. Controlled delivery of non-steroidal anti-inflammatory drugs to the repair site via an implanted scaffold is a promising option for modulating inflammation in the healing environment. Furthermore, biodegradable nanofibrous delivery systems offer an optimized architecture and surface area for cellular attachment, proliferation, and infiltration while releasing soluble factors to promote tendon regeneration. To this end, we developed a bilayer delivery system (BiLDS) for localized and controlled release of ibuprofen (IBP) to temporally mitigate inflammation and enhance tendon remodeling following surgical repair by promoting organized tissue formation. In vitro evaluation confirmed the delayed and sustained release of IBP from Labrafil-modified poly(lactic-co-glycolic) acid microspheres within sintered poly(ε-caprolactone) electrospun scaffolds. Biocompatibility of the BiLDS was demonstrated with primary Achilles tendon cells in vitro. Implantation of the IBP-releasing BiLDS at the repair site in a rat rotator cuff injury and repair model led to decreased expression of proinflammatory cytokine, tumor necrotic factor-α, and increased anti-inflammatory cytokine, transforming growth factor-β1. The BiLDS remained intact for mechanical reinforcement and recovered the tendon structural properties by 8 weeks. These results suggest the therapeutic potential of a novel biocompatible nanofibrous BiLDS for localized and tailored delivery of IBP to mitigate tendon inflammation and improve repair outcomes. Future studies are required to define the mechanical implications of an optimized BiLDS in a rat model beyond 8 weeks or in a larger animal model.<br /> (© 2020 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.)

Details

Language :
English
ISSN :
1554-527X
Volume :
38
Issue :
11
Database :
MEDLINE
Journal :
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Publication Type :
Academic Journal
Accession number :
32215953
Full Text :
https://doi.org/10.1002/jor.24670