Back to Search
Start Over
Pyoverdine-Mediated Killing of Caenorhabditis elegans by Pseudomonas syringae MB03 and the Role of Iron in Its Pathogenicity.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2020 Mar 22; Vol. 21 (6). Date of Electronic Publication: 2020 Mar 22. - Publication Year :
- 2020
-
Abstract
- The pathogenicity of the common phytopathogenic bacterium Pseudomonas syringae toward Caenorhabditis elegans has been recently demonstrated. However, the major virulence factors involved in this interaction remain unknown. In this study, we investigated the nematocidal activity of P. syringae against C. elegans under iron-sufficient/limited conditions, primarily focusing on the role of the ferric chelator pyoverdine in a P. syringae - C. elegans liquid-based pathogenicity model. Prediction-based analysis of pyoverdine-encoding genes in the genome of the wild-type P. syringae strain MB03 revealed that the genes are located in one large cluster. Two non-ribosomal peptide synthetase genes ( pvdD and pvdJ ) were disrupted via a Rec/TE recombination system, resulting in mutant strains with abrogated pyoverdine production and attenuated virulence against C. elegans. When used alone, pure pyoverdine also showed nematocidal activity. The role of iron used alone or with pyoverdine was further investigated in mutant and MB03-based bioassays. The results indicated that pyoverdine in P. syringae MB03 is a robust virulence factor that promotes the killing of C. elegans . We speculate that pyoverdine functions as a virulence determinant by capturing environmentally available iron for host bacterial cells, by limiting its availability for C. elegans worms, and by regulating and/or activating other intracellular virulence factors that ultimately kills C. elegans worms.
- Subjects :
- Animals
Bacterial Proteins genetics
Caenorhabditis elegans physiology
Genome, Bacterial
Host-Pathogen Interactions
Oligopeptides genetics
Pseudomonas syringae genetics
Pseudomonas syringae physiology
Siderophores genetics
Siderophores metabolism
Virulence Factors genetics
Virulence Factors metabolism
Bacterial Proteins metabolism
Caenorhabditis elegans microbiology
Iron metabolism
Oligopeptides metabolism
Pseudomonas syringae pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 21
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 32235814
- Full Text :
- https://doi.org/10.3390/ijms21062198