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The translational landscape of ground state pluripotency.
- Source :
-
Nature communications [Nat Commun] 2020 Apr 01; Vol. 11 (1), pp. 1617. Date of Electronic Publication: 2020 Apr 01. - Publication Year :
- 2020
-
Abstract
- Translational control plays a central role in regulation of gene expression and can lead to significant divergence between mRNA- and protein-abundance. Here, we used genome-wide approaches combined with time-course analysis to measure the mRNA-abundance, mRNA-translation rate and protein expression during the transition of naïve-to-primed mouse embryonic stem cells (ESCs). We find that the ground state ESCs cultured with GSK3-, MEK-inhibitors and LIF (2iL) display higher ribosome density on a selective set of mRNAs. This set of mRNAs undergo strong translational buffering to maintain stable protein expression levels in 2iL-ESCs. Importantly, we show that the global alteration of cellular proteome during the transition of naïve-to-primed pluripotency is largely accompanied by transcriptional rewiring. Thus, we provide a comprehensive and detailed overview of the global changes in gene expression in different states of ESCs and dissect the relative contributions of mRNA-transcription, translation and regulation of protein stability in controlling protein abundance.
- Subjects :
- Animals
Gene Expression Regulation
Glycogen Synthase Kinase 3 metabolism
Metabolic Networks and Pathways
Mice
Mouse Embryonic Stem Cells metabolism
Polyribosomes genetics
Ribosomes genetics
Transcriptome
Embryonic Stem Cells metabolism
Polyribosomes metabolism
Proteome metabolism
RNA, Messenger metabolism
Ribosomes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 32238817
- Full Text :
- https://doi.org/10.1038/s41467-020-15449-9