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Broad-spectrum detection of zeranol and its analogues by a colloidal gold-based lateral flow immunochromatographic assay in milk.
- Source :
-
Food chemistry [Food Chem] 2020 Aug 15; Vol. 321, pp. 126697. Date of Electronic Publication: 2020 Mar 26. - Publication Year :
- 2020
-
Abstract
- Based on colloidal gold and broad-spectrum monoclonal antibody that binds to zeranol and its five analogues with high sensitivity, a lateral flow immunochromatographic assay (LFIA) in a competitive format was developed to specifically determine residues of zeranol, an illegal growth promoter in livestock. In this study, the assay had high sensitivity and was broad-spectrum only for zeranol and its five analogues, and the results were obtained within 10 min without needing sophisticated procedures. The cutoff values for zeranol and its five analogues were 10 ng/mL, and the IC <subscript>50</subscript> values for zeranol, β-zearalanol, zearalanone, α-zearalenol, β-zearalenol and zearalenone were 1.250, 1.800, 1.775, 1.225, 1.709 and 1.319 ng/mL, respectively. The recovery rates were ranged from 85.6 to 93.9%, with the coefficient of variations less than 12.4%. The results demonstrated that the LFIA could be used for rapid, simultaneous, semi-quantitative and quantitative detection of residues of zeranol and its five analogous in milk.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibodies, Monoclonal immunology
Equipment Design
Food Analysis instrumentation
Gold Colloid chemistry
Immunoassay instrumentation
Sensitivity and Specificity
Zearalenone analysis
Zeranol analogs & derivatives
Zeranol immunology
Food Analysis methods
Food Contamination analysis
Immunoassay methods
Milk chemistry
Zeranol analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-7072
- Volume :
- 321
- Database :
- MEDLINE
- Journal :
- Food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32244141
- Full Text :
- https://doi.org/10.1016/j.foodchem.2020.126697