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Multicenter flow cytometry proficiency testing of canine blood and lymph node samples.
- Source :
-
Veterinary clinical pathology [Vet Clin Pathol] 2020 Jun; Vol. 49 (2), pp. 249-257. Date of Electronic Publication: 2020 Apr 04. - Publication Year :
- 2020
-
Abstract
- Background: Flow cytometry (FC) is used increasingly in veterinary medicine for further characterization of hematolymphoid cells. Guidelines for optimizing assay performance and interpretation of results are limited, and concordance of results across laboratories is unknown.<br />Objectives: This study aimed to determine inter-investigator agreement on the interpretation of FC results from split samples analyzed in different laboratories using various protocols, cytometers, and software; and on the interpretation of archived FC standard (FCS) data files contributed by the different investigators.<br />Methods: This was a multicenter observational cross-sectional study. Anticoagulated blood or lymph node aspirate samples from nine client-owned dogs were aliquoted and shipped to participating laboratories. Samples were analyzed with individual laboratory-developed protocols. In addition, FCS files from a set of separate samples from 11 client-owned dogs were analyzed by participating investigators. A person not associated with the study tabulated the results and interpretations. Agreement of interpretations was assessed with Fleiss' kappa statistic.<br />Results: Prolonged transit times affected sample quality for some laboratories. Overall agreement among investigators regarding the FC sample interpretation was strong (κ = 0.86 ± 0.19, P < .001), and for specific categories, ranged from moderate to perfect. Agreement of the lymphoproliferation or other leukocyte sample category from the analysis of the FCS files was weak (κ = 0.58 ± 0.05, P < .001).<br />Conclusions: Lymphoproliferations were readily identified by FC, but identification of the categories of hematolymphoid neoplasia in fresh samples or archived files was variable. There is a need for a more standardized approach to maximize the enormous potential of FC in veterinary medicine.<br /> (© 2020 American Society for Veterinary Clinical Pathology.)
- Subjects :
- Animals
Cross-Sectional Studies
Dog Diseases blood
Dog Diseases pathology
Dogs
Flow Cytometry standards
Hematologic Neoplasms diagnosis
Hematologic Neoplasms pathology
Immunophenotyping veterinary
Laboratory Proficiency Testing standards
Lymph Nodes pathology
Lymphoproliferative Disorders diagnosis
Lymphoproliferative Disorders pathology
Dog Diseases diagnosis
Flow Cytometry veterinary
Hematologic Neoplasms veterinary
Lymphoproliferative Disorders veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1939-165X
- Volume :
- 49
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Veterinary clinical pathology
- Publication Type :
- Academic Journal
- Accession number :
- 32246538
- Full Text :
- https://doi.org/10.1111/vcp.12843