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An ultrasensitive electrochemical immunosensor for CEA using MWCNT-NH 2 supported PdPt nanocages as labels for signal amplification.
- Source :
-
Journal of materials chemistry. B [J Mater Chem B] 2015 Mar 07; Vol. 3 (9), pp. 2006-2011. Date of Electronic Publication: 2015 Jan 30. - Publication Year :
- 2015
-
Abstract
- The excellent catalytic activity of a novel nanomaterial was introduced to ultrasensitive sandwich-type electrochemical immunosensor applications for detection of carcinoembryonic antigen (CEA). Amino-functionalized multi-walled carbon nanotube (MWCNT-NH <subscript>2</subscript> ) supported PdPt nanocages were used as catalytic labels of secondary anti-CEA. PdPt nanocages with hollow interiors and porous walls could efficiently enhance electrocatalysis for reduction of H <subscript>2</subscript> O <subscript>2</subscript> . Meanwhile, with good dispersion, large specific surface area and amino groups, MWCNT-NH <subscript>2</subscript> can immobilize a large amount of PdPt nanocages to improve the sensitivity and amplify the signal of the immunosensor. For the immobilization of primary antibodies, 3-aminopropyltriethoxysilane functionalized graphene sheets were used as transducing materials to modify glassy carbon electrodes and to enhance the electron transfer efficiently. Under the optimal conditions, the electrochemical immunosensor exhibited a wide linear range of 0.001-20 ng mL <superscript>-1</superscript> with a low detection limit of 0.2 pg mL <superscript>-1</superscript> for CEA. The proposed sensing strategy enriches electrochemical immunoassays and may have a promising application in bioassay analysis.
Details
- Language :
- English
- ISSN :
- 2050-7518
- Volume :
- 3
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of materials chemistry. B
- Publication Type :
- Academic Journal
- Accession number :
- 32262270
- Full Text :
- https://doi.org/10.1039/c4tb01695e