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Enhancement of Subcutaneously Transplanted β Cell Survival Using 3D Stem Cell Spheroids with Proangiogenic and Prosurvival Potential.
- Source :
-
Advanced biosystems [Adv Biosyst] 2020 Mar; Vol. 4 (3), pp. e1900254. Date of Electronic Publication: 2020 Feb 10. - Publication Year :
- 2020
-
Abstract
- Islet transplantation has been demonstrated to be a promising therapy for type 1 diabetes mellitus. Although it is a minimally invasive operating procedure and provides easy access for graft monitoring, subcutaneous transplantation of the islet only has limited therapeutic outcomes, owing to the poor capacity of skin tissue to foster revascularization in a short period. Herein, 3D cell spheroids of clinically accessible umbilical cord blood mesenchymal stem cells and human umbilical vein endothelial cells are formed and employed for codelivery with β cells subcutaneously. The 3D stem cell spheroids, which can secrete multiple proangiogenic and prosurvival growth factors, induce robust angiogenesis and prevent β cell graft death, as indicated by the results of in vivo bioluminescent tracking and histological analysis. These experimental data highlight the efficacy of the 3D stem cell spheroids that are fabricated using translationally applicable cell types in promoting the survival and function of subcutaneously transplanted β cells.<br /> (© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Animals
Cells, Cultured
Human Umbilical Vein Endothelial Cells cytology
Human Umbilical Vein Endothelial Cells transplantation
Humans
Mesenchymal Stem Cells cytology
Mice
Mice, Nude
Cell Survival physiology
Insulin-Secreting Cells physiology
Insulin-Secreting Cells transplantation
Neovascularization, Physiologic physiology
Spheroids, Cellular cytology
Spheroids, Cellular transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 2366-7478
- Volume :
- 4
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Advanced biosystems
- Publication Type :
- Academic Journal
- Accession number :
- 32293147
- Full Text :
- https://doi.org/10.1002/adbi.201900254