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Biochemical analysis of cellobiose catabolism in Candida pseudointermedia strains isolated from rotten wood.
- Source :
-
Archives of microbiology [Arch Microbiol] 2020 Sep; Vol. 202 (7), pp. 1729-1739. Date of Electronic Publication: 2020 Apr 23. - Publication Year :
- 2020
-
Abstract
- We isolated two Candida pseudointermedia strains from the Atlantic rain forest in Brazil, and analyzed cellobiose metabolization in their cells. After growth in cellobiose medium, both strains had high intracellular β-glucosidase activity [~ 200 U (g cells) <superscript>-1</superscript> for 200 mM cellobiose and ~ 100 U (g cells) <superscript>-1</superscript> for 2 mM pNPβG] and negligible periplasmic cellobiase activity. During batch fermentation, the strain with the best performance consumed all the available cellobiose in the first 18 h of the assay, producing 2.7 g L <superscript>-1</superscript> of ethanol. Kinetics of its cellobiase activity demonstrated a high-affinity hydrolytic system inside cells, with K <subscript>m</subscript> of 12.4 mM. Our data suggest that, unlike other fungal species that hydrolyze cellobiose extracellularly, both analyzed strains transport it to the cytoplasm, where it is then hydrolyzed by high-affinity intracellular β-glucosidases. We believe this study increases the fund of knowledge regarding yeasts from Brazilian microbiomes.
Details
- Language :
- English
- ISSN :
- 1432-072X
- Volume :
- 202
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Archives of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 32328754
- Full Text :
- https://doi.org/10.1007/s00203-020-01884-1